Intralipid 10%:: Physicochemical characterization
Intralipid 10%:: Physicochemical characterization
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DOI：
10.1016/s0899-9007(01)00667-0
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发表时间：
2001-11-01
期刊：
影响因子：
4.4
通讯作者：
Bach, AC
中科院分区：
文献类型：
--
作者：
Férézou, J;Gulik, A;Bach, AC
目的：肠外脂肪乳含有两种颗粒：富含三酰甘油（TAG）的人工乳糜微粒和脂质体（包裹水相的双层磷脂[PL]）。离心可以分离婴儿中称为中间期的脂质体。本工作的目的是更好地表征这种中间相的化学性质，并通过电子显微镜观察它所含的粒子。方法：将天然10%脂内、中间相（在27000 g下离心1小时）和脂质体富集部分（中间相在10 000 g下KBr密度梯度离心24小时后得到密度为1.010-1.030 g/l的环）冷冻后进行电镜观察（Philips 410）。分析中间期TAG和蛋白含量，免疫检测（Western-blot）对蛋白进行部分表征。结果：10%脂内的电镜研究表明，人工乳糜微粒（平均直径260 nm）和脂质体（43 nm）共存，脂质体比预期的要小，纯化后含有8% w/w的TAG。在肠外乳剂中，TAG在PL双分子层中的增溶作用（据报道小于或等于3.1% w/w）可能由于制造工艺或高TAG/PL比而增加。少量的蛋白质也被检测到，并使用针对人7 kDa阴离子多肽因子（APF）的特异性抗体进行部分表征，已知APF与胆汁中的PL强烈相互作用。结论：本研究结果表明，该方法可有效地提高肿瘤细胞的平均直径。43 nm)的脂质体比通常认为的要小。乳剂中疏水蛋白的痕量可以解释有时在输注患者中观察到的某些过敏反应。(C) Elsevier Science Inc., 2001。
OBJECTIVES: Parenteral fat emulsions contain two populations of particles: artificial chylomicrons rich in triacylglycerols (TAG), and liposomes (bilayer of phospholipids [PL] enveloping an aqueous phase). Centrifugation permits isolating the liposomes in the infranatant called mesophase. The aim of the present work was to better characterize this mesophase chemically and to view the particles it contains by electron microscopy.METHODS: Electron microscopy (Philips 410) was performed after cryofracture on native 10% Intralipid, mesophase (centrifugation for 1 h at 27 000 g), and a liposome-enriched fraction (ring of density 1.010-1.030 g/l obtained after centrifuging mesophase in a KBr density gradient at 100 000 g for 24 h). The TAG and protein content of the mesophase was analyzed and the proteins partially characterized by immunodetection (Western-blot).RESULTS: This electron microscope study of 10% Intralipid gives evidence for the coexistence of artificial chylomicrons (mean diameter, 260 nm) and liposomes (43 nm), the latter being smaller than expected and containing 8% w/w TAG after purification. The solubilization of TAG in PL bilayers (reported to be less than or equal to 3.1% w/w) might have been increased in parenteral emulsions by the manufacturing process or/and the high TAG/PL ratio. Minute amounts of proteins have also been detected and partially characterized using a specific antibody raised against the human 7 kDa Anionic Polypeptide Factor (APF), known to strongly interact with PL in bile.CONCLUSIONS: This work has shown that the size (mean diameter. 43 nm) of the liposomes present in 10% Intralipid is smaller than that usually assumed. Traces of hydrophobic proteins in the emulsion may account for certain allergic reactions sometimes observed in infused patients. (C) Elsevier Science Inc. 2001.