A novel subunit for Shal K+ channels radically alters activation and inactivation
A novel subunit for Shal K+ channels radically alters activation and inactivation
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DOI：
10.1523/jneurosci.17-01-00032.1997
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发表时间：
1997-01-01
影响因子：
5.3
通讯作者：
Salkoff, L
中科院分区：
文献类型：
--
作者：
Jegla, T;Salkoff, L
Shal (Kv4) 钾通道基因编码经典阈下 A 电流，其调节可能是决定神经元放电频率的关键因素。果蝇和哺乳动物中目前尚未鉴定的一类蛋白质会增加 Shal 通道的失活率。我们从水母 Polyorchis penicillatus 中克隆了一种新型 Shal 通道亚基（jShal gamma 1），它可以改变无脊椎动物和脊椎动物的 Shal 电流。当与保守的水母 Shal 同源物 jShal1 共表达时，jShal gamma 1 显着改变失活速率以及激活和稳态失活的电压范围，jShal gamma 1 通过经典的 N 型机制提供快速失活，这与其对电压依赖性的影响无关，jShal gamma 1 仅作为异多聚体形成功能通道，jShal gamma 1 + jShal1 异多聚体仅在2:2 亚基化学计量。
Shal (Kv4) potassium channel genes encode classical subthreshold A-currents, and their regulation may be a key factor in determining neuronal firing frequency. The inactivation rate of Shal channels is increased by a presently unidentified class of proteins in both Drosophila and mammals. We have cloned a novel Shal channel subunit (jShal gamma 1) from the jellyfish Polyorchis penicillatus that alters Shal currents from both invertebrates and vertebrates. When co-expressed with the conserved jellyfish Shal homolog jShal1, jShal gamma 1 dramatically changes both the rate of inactivation and voltage range of activation and steady-state inactivation, jShal gamma 1 provides fast inactivation by a classic N-type mechanism, which is independent of its effects on voltage dependence, jShal gamma 1 forms functional channels only as a heteromultimer, and jShal gamma 1 + jShal1 heteromultimers are functional only in a 2:2 subunit stoichiometry.