Strategies for in vitro engineering of the translation machinery
Strategies for in vitro engineering of the translation machinery
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DOI：
10.1093/nar/gkz1011
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发表时间：
2020-02-20
影响因子：
14.9
通讯作者：
Jewett, Michael C.
中科院分区：
文献类型：
--
作者：
Hammerling, Michael J.;Kruger, Antje;Jewett, Michael C.
分子翻译或蛋白质生物合成过程的工程化已经成为合成和化学生物学中产生新的生物学见解并实现新应用（例如设计蛋白质治疗）的主要机会。在这里，我们回顾了体外翻译过程的工程方法。我们讨论了两种主要策略的优点和缺点，纯化和提取为基础的系统，以及如何使用它们来操纵和研究翻译。工程技术的翻译机器的每个组成部分依次涵盖，包括转移RNA，翻译因子和核糖体。最后，未来的方向和使该领域的技术进步进行了讨论。
Engineering the process of molecular translation, or protein biosynthesis, has emerged as a major opportunity in synthetic and chemical biology to generate novel biological insights and enable new applications (e.g. designer protein therapeutics). Here, we review methods for engineering the process of translation in vitro. We discuss the advantages and drawbacks of the two major strategies-purified and extract-based systems-and how they may be used to manipulate and study translation. Techniques to engineer each component of the translation machinery are covered in turn, including transfer RNAs, translation factors, and the ribosome. Finally, future directions and enabling technological advances for the field are discussed.