Deuterated hen egg-white lysozyme crystals: Optimization of the growth conditions and morphology
Deuterated hen egg-white lysozyme crystals: Optimization of the growth conditions and morphology
复制标题
氘代鸡蛋清溶菌酶晶体：生长条件和形态的优化
DOI：
10.1134/1.2049397
复制
发表时间：
2005
影响因子：
0.7
通讯作者：
L. A. Shuvalov
中科院分区：
文献类型：
--
作者：
A. Svanidze;S. Lushnikov;L. A. Shuvalov
Deuterated and protonated tetragonal lysozyme crystals are grown using the hanging-drop vapor-diffusion method. The size of the lysozyme crystals grown is determined as a function of the concentration of sodium chloride used as a precipitant. It is found that crystallization leads to the formation of lysozyme crystals with three different habits. Morphological and X-ray diffraction analyses of the deuterated and protonated lysozyme crystals demonstrate that, despite the different habits, all the crystals grown belong to the tetragonal crystal system. The simple forms of lysozyme crystals are revealed. It is shown that the habits of the lysozyme crystals are determined by the specific combinations of simple forms. The mechanisms responsible for the formation of lysozyme crystals with different habits are discussed.
