[The Effect of Morusin on Stemness Phenotype of Laryngeal Cancer Stem Cell].
[The Effect of Morusin on Stemness Phenotype of Laryngeal Cancer Stem Cell].
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DOI：
10.12182/20200960503
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发表时间：
2020-09-01
期刊：
影响因子：
--
通讯作者：
Wang, Li
中科院分区：
文献类型：
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作者：
Chen, Chen;Xu, Zi-Han;Wang, Li
目的：方法：采用流式细胞仪分离检测CD 133+喉癌干细胞的比例，肿瘤球形成实验检测CD 133+喉癌干细胞的自我更新能力，Transwell实验检测CD 133+喉癌干细胞的迁移能力，流式细胞仪检测CD 133+喉癌干细胞的增殖能力，流式细胞仪检测CD 133+喉癌干细胞的增殖能力。采用改良MTT法分析化疗药物对CD 133+喉癌干细胞的细胞毒作用;采用免疫荧光染色、RT-qPCR和Western blot检测干细胞相关标志物的表达水平。用不同浓度的Morusin处理细胞后，进行上述实验，检测细胞的自我更新能力、迁移能力、细胞毒性抗性以及干细胞相关标志物的表达。流式细胞仪分析显示，CD 133+喉癌干细胞的比例为（3.50±0.34）%，而富集后比例增加到（93.20±5.23）%。CD 133+喉癌干细胞具有较强的自我更新能力（P
OBJECTIVE: To investigate the regulation effect of Morusin on stemness phenotype of laryngeal cancer stem cells.METHODS: Separation and detection the proportion of CD133 + laryngeal cancer stem cells through flow cytometry; evaluation the self-renewal ability of CD133 + laryngeal cancer stem cells by tumor sphere formation assay; exploring the migration ability of CD133 + laryngeal cancer stem cells by Transwell assay; analyzing the cytotoxicity of chemotherapy drugs on CD133 + laryngeal cancer stem cells by modified MTT assay; detection of the expression levels of stemness associated markers by immunofluorescence staining, RT-qPCR and Western blot. After treatment with different concentrations of Morusin, cells were performed the above experiments for detection the self-renewal ability, migration ability, cytotoxicity resistance and expression of stemness associated markers.RESULTS: Flow cytometry analysis showed that the proportion of CD133 + laryngeal cancer stem cells was (3.50±0.34)%, while after enrichment, the proportion increased to (93.20±5.23)%. CD133 + laryngeal cancer stem cells exhibited better self-renewal ability (P