OsGLO4 is involved in the formation of iron plaques on surface of rice roots grown under alternative wetting and drying condition
OsGLO4 is involved in the formation of iron plaques on surface of rice roots grown under alternative wetting and drying condition
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OsGLO4 参与交替干湿条件下生长的水稻根部表面铁斑的形成
DOI：
10.1007/s11104-017-3493-5
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发表时间：
2018-02
期刊：
影响因子：
--
通讯作者：
Hong Shen
中科院分区：
文献类型：
--
作者：
Xiao-Li Yu;Dao-Ming Wu;You-Qiang Fu;Xu-Jian Yang;František Baluška;Hong Shen
背景与目的交替干湿(AWD)促进铁斑形成的分子生理机制尚不清楚。在此，我们报道了乙醇酸氧化酶(GLO)基因OsGLO4参与了AWD诱导的IP的形成。方法在沙地上种植水稻植株，探讨AWD处理下IP含量、GLO活性、H_2O_2浓度和OsGLO4表达之间的关系。结果AWD显著促进水稻根部GLO活性、OsGLO4表达和IP含量。过表达的OsGLO4(OX)的IP含量比野生型(WT)高46.2%。X-射线衍射分析结果表明，与WT相比，OX在IP中也含有更多的FeIII化合物。牛根的GLO活性和H_2O_2浓度分别比对照高170.3%和126.7%。有趣的是，H_2O_2在表皮细胞中的分布与IP的空间分布一致。乙醇酸处理可使GLO活性提高88.9%，IP含量增加33.3%，而α-羟基-2-吡啶甲磺酸则抑制GLO活性，降低IP含量。外源H_2O_2或氨基三氮唑处理可显著增加IP的生成，而二甲基硫脲则显著减少IP的生成。结论OsGLO4可能通过调节AWD条件下GLO活性和H_2O_2的产生参与IP的形成。
Background and aimThe molecular physiological mechanism of alternative wetting and drying (AWD) promoting iron plaque (IP) formation is unknown. Here, we report that a glycolate oxidase (GLO) gene, OsGLO4 contributes to AWD-induced IP formation.MethodsRice plants were grown in sand to explore the relationship among IP content, GLO activity, H2O2 concentration and OsGLO4 expression under AWD treatment.ResultsAWD significantly promoted GLO activity, OsGLO4 expression,and IP content in rice roots. Overexpressing OsGLO4 (OX) showed more 46.2% IP content than wild type (WT). Results with X-ray diffraction analysis indicated that OX also had a higher proportion of FeIII compound in IP in comparison to WT. The activity of GLO and H2O2 concentration in OX roots were 170.3% and 126.7% higher than those of WT. Interestingly, H2O2 distribution in epidermal cells was consistent with the spatial distribution of IP. Treatment with glycolic acid raised GLO activity by 88.9% and IP content by 33.3%; while α-hydroxy-2-pyridinemethanesulfonic acid inhibited GLO activity and reduced IP content. Exogenous H2O2 or treatment with aminotriazole increased the quantities of IP significantly, while dimethylthiourea reduced it.ConclusionOsGLO4 is involved in the formation of IP by mediating GLO activity and H2O2 production under AWD condition.
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影响因子：
6.9
作者：
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DOI：
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发表时间：
1971
期刊：
--
影响因子：
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作者：
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通讯作者：
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影响因子：
3.7
作者：
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