DISTRIBUTION OF D2-DOPAMINE RECEPTOR MESSENGER-RNA IN RAT-BRAIN
DISTRIBUTION OF D2-DOPAMINE RECEPTOR MESSENGER-RNA IN RAT-BRAIN
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DOI：
10.1073/pnas.86.19.7625
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发表时间：
1989-10-01
影响因子：
11.1
通讯作者：
CIVELLI, O
中科院分区：
文献类型：
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作者：
MEADORWOODRUFF, JH;MANSOUR, A;CIVELLI, O
用原位杂交技术研究了多巴胺D2受体mRNA在大鼠脑内的分布。对应于大鼠D2受体的推定的第三胞质环和第六和第七跨膜结构域的cDNA探针用于产生35S标记的核糖核酸探针以与D2受体mRNA杂交。D2 mRNA被发现在多巴胺投射领域，并在与多巴胺的细胞体，这表明突触后和突触前自身受体定位的区域。最高浓度的D2 mRNA被发现在新纹状体，嗅结节，黑质，腹侧被盖区，和延髓核。该分布与D2受体放射自显影术报告的分布一致。
The distribution of mRNA coding for the D2 dopamine receptor was studied in the rat brain by in situ hybridization. A cDNA probe corresponding to the putative third cytosolic loop and sixth and seventh transmembrane domains of the rat D2 receptor was used to generate an 35S-labeled riboprobe to hybridize to D2 receptor mRNA. D2 mRNA was found both in dopamine projection fields and in regions associated with dopamine-containing cell bodies, suggesting both postsynaptic and presynaptic autoreceptor localization. Highest concentrations of D2 mRNA were found in neostriatum, olfactory tubercle, substantia nigra, ventral tegmental area, and the nucleus accumbens. This distribution is consistent with those reported with D2 receptor autoradiography.