Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose.
Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose.
复制标题
大肠杆菌从葡萄糖生产 (2S,3S)-丁烷-2,3-二醇的代谢工程
DOI：
10.1186/s13068-015-0324-x
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发表时间：
2015
影响因子：
6.3
通讯作者：
Gao C
中科院分区：
文献类型：
--
作者：
Chu H;Xin B;Liu P;Wang Y;Li L;Liu X;Zhang X;Ma C;Xu P;Gao C
2,3 - 丁二醇（2,3 - BD）是一种燃料和平台生化物质，具有多种工业应用。2,3 - BD存在三种立体异构形式：（2R,3R）- 2,3 - BD、内消旋 - 2,3 - BD和（2S,3S）- 2,3 - BD。已有微生物发酵工艺用于（2R,3R）- 2,3 - BD和内消旋 - 2,3 - BD的生产。
通过共表达阴沟肠杆菌溶解亚种SDM菌株的α - 乙酰乳酸合酶和内消旋 - 2,3 - 丁二醇脱氢酶的重组大肠杆菌全细胞，实现了从葡萄糖生产（2S,3S）- 2,3 - BD。构建了一种用于生产（2S,3S）- 2,3 - BD的最佳生物催化剂——大肠杆菌BL21（pETDuet - PT7 - budB - PT7 - budC），并对生物转化条件进行了优化。在生物转化系统中添加10 mM氯化铁时，利用代谢工程菌株从葡萄糖可获得浓度为2.2 g/L的（2S,3S）- 2,3 - BD，其立体异构纯度为95.0%。
该工程大肠杆菌菌株是首个可用于从葡萄糖直接生产（2S,3S）- 2,3 - BD的菌株。结果表明，此处开发的方法对于高效生产（2S,3S）- 2,3 - BD将是一个有前景的工艺。
本文的网络版（doi:10.1186/s13068 - 2015 - 0324 - x）包含补充材料，授权用户可获取。
Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production. The production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the α-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet–PT7–budB–PT7–budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose. The engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production. The online version of this article (doi:10.1186/s13068-015-0324-x) contains supplementary material, which is available to authorized users.