Neutrophils Are More Effective than Monocytes at Phagosomal Containment and Killing of Listeria monocytogenes.
Neutrophils Are More Effective than Monocytes at Phagosomal Containment and Killing of Listeria monocytogenes.
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DOI：
10.4049/immunohorizons.1900065
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发表时间：
2019-12-13
期刊：
影响因子：
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通讯作者：
Berg, Rance E
中科院分区：
文献类型：
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作者：
Okunnu, Busola M;Berg, Rance E
中性粒细胞和炎性单核细胞是单核细胞增多性李斯特菌感染期间保护所必需的先天免疫细胞。虽然某些功能通常被分配给每个细胞，但细菌清除所需功能的相似性和差异以前尚未被研究过。在当前的研究中，研究了单核细胞增多性李斯特菌感染期间嗜中性粒细胞和单核细胞的吞噬作用、吞噬体遏制、细菌杀灭以及细胞因子的产生。通过体外研究获得的数据表明，中性粒细胞在摄取单核细胞增多性李斯特菌、吞噬体遏制和杀伤方面比单核细胞更有效。然而，在体内单核细胞增生李斯特菌感染期间，单核细胞在细胞因子产生方面更有效。此外，数据表明，中性粒细胞和单核细胞也能够产生 IL-1α，这是一种细胞因子，在单核细胞增多性李斯特菌感染期间尚未明确定义其作用。此外，我们能够证明单核细胞群体同时产生 TNF-α 和 IL-α。这项研究强调了中性粒细胞和单核细胞的多功能能力，进一步增加了我们对单核细胞增多性李斯特菌感染期间这些先天免疫细胞的了解。
Neutrophils and inflammatory monocytes are innate immune cells essential for protection during Listeria monocytogenes infection. Although certain functions have been generally assigned to each of the cells, similarities and differences in functions necessary for bacterial clearance have not previously been investigated. In the current study, phagocytosis, phagosomal containment, bacterial killing, and cytokine production by neutrophils and monocytes during L. monocytogenes infection were studied. Data obtained via in vitro studies show that neutrophils are more effective at L. monocytogenes uptake, phagosomal containment, and killing than monocytes. However, monocytes were found to be more effective at cytokine production during L. monocytogenes infection, in vivo. Additionally, the data demonstrated that neutrophils and monocytes are also capable of producing IL-1alpha, a cytokine that does not yet have a clearly defined role during infection with L. monocytogenes Furthermore, we were able to demonstrate a population of monocytes producing both TNF-alpha and IL-alpha, concurrently. This study highlights the multifunctional capabilities of neutrophils and monocytes, further adding to our knowledge of these innate immune cells during L. monocytogenes infection.