INTERNAL CONTROL REGIONS FOR TRANSCRIPTION OF EUKARYOTIC TRANSFER-RNA GENES
INTERNAL CONTROL REGIONS FOR TRANSCRIPTION OF EUKARYOTIC TRANSFER-RNA GENES
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DOI：
10.1073/pnas.78.11.6657
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
SOLL, D
中科院分区：
文献类型：
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作者：
SHARP, S;DEFRANCO, D;SOLL, D
鉴定了真核tRNA基因内起始转录所需的区域。这些结果是通过使用核酸酶bal31在克隆的果蝇tRNAArg基因中系统地构建从5‘或3’侧翼区域延伸的缺失得到的。在非洲爪蟾卵母细胞、果蝇Kc细胞和HeLa细胞的转录系统中，测量了新生成的缺失克隆对tRNA前体体外合成的指导能力。在编码序列中确定了两个控制区域。第一个对转录至关重要，位于成熟tRNA序列的核苷酸8和25之间。第二个控制区（位于成熟tRNA序列的核苷酸50和58之间）缺失的基因可以转录，但效率降低。tRNA基因的启动子区域编码D茎和D环的tRNA序列、8位不变的尿苷和半不变的G-T-.psi。- c序列。
The region within a eukaryotic tRNA gene required for initiation of transcription was identified. These results were obtained by systematically constructing deletions extending from the 5'' or the 3'' flanking regions into a cloned Drosophila tRNAArg gene by using nuclease BAL 31. The ability of the newly generated deletion clones to direct the in vitro synthesis of tRNA precursors was measured in transcription systems from Xenopus laevis oocytes, Drosophila Kc cells and HeLa cells. Two control regions within the coding sequence were identified. The 1st was essential for transcription and was contained between nucleotides 8 and 25 of the mature tRNA sequence. Genes devoid of the 2nd control region, which was contained between nucleotides 50 and 58 of the mature tRNA sequence, could be transcribed but with reduced efficiency. The promoter regions within a tRNA gene encode the tRNA sequences of the D stem and D loop, the invariant uridine at position 8, and the semi-invariant G-T-.psi.-C sequence.