A novel method for the measurement of plasma metanephrines using online solid phase extraction-liquid chromatography tandem mass spectrometry
A novel method for the measurement of plasma metanephrines using online solid phase extraction-liquid chromatography tandem mass spectrometry
复制标题
DOI：
10.1177/0004563214546560
复制
发表时间：
2015-05-01
影响因子：
2.2
通讯作者：
Keevil, Brian G.
中科院分区：
文献类型：
--
作者：
Adaway, Joanne E.;Peitzsch, Mirko;Keevil, Brian G.
背景血浆间甲肾上腺素、去甲间甲肾上腺素和3-甲氧基酪胺的测定在嗜铬细胞瘤的诊断中是有用的，但是由于测定灵敏度差，许多测定需要大量的血浆，并且通常需要长时间的样品制备。我们的目的是开发一种方法，用于测量血浆变肾上腺素使用一个小的样品体积与最小的动手preparation.Methods样品进行脱蛋白使用10 K旋转过滤器，然后使用沃茨Acquity UPLC在线固相萃取管理器（沃茨，曼彻斯特，英国）耦合到一个沃茨Xevo TQ-S质谱仪（沃茨，曼彻斯特，英国）。该方法经过验证，结果相比，以前发表的method.Results我们实现了定量限为37.5pmol/L的metanetamine和3-甲氧基酪胺和75 pmol/L的normetanetamine使用只有150 μ L的样品。该方法的线性高达30，000 pmol/L的所有分析物，并在方法比较研究的结果显示良好的协议与以前发表的LC-MS/MS assay.Conclusions，我们已经开发出一种简单的方法，用于测量血浆metaneurine，normetaneurine和3-甲氧基酪胺，仅使用150 μ L的样品。需要最少的动手样品制备，并且该测定适合于临床实验室的常规使用。
Background Measurement of plasma metanephrine, normetanephrine and 3-methoxytyramine is useful in the diagnosis of phaeochromocytomas, but many assays require a large volume of plasma due to poor assay sensitivity, and often require lengthy sample preparation. Our aim was to develop a method for measurement of plasma metanephrines using a small sample volume with minimal hands-on preparation.Methods Samples were deproteinised using 10K spin filters prior to online solid phase extraction using a Waters Acquity UPLC Online SPE Manager (Waters, Manchester, UK) coupled to a Waters Xevo TQ-S mass spectrometer (Waters, Manchester, UK). The assay was validated and results compared to a previously published method.Results We achieved a limit of quantification of 37.5pmol/L for metanephrine and 3-methoxytyramine and 75pmol/L for normetanephrine using only 150 mu L of sample. The assay was linear up to 30,000pmol/L for all analytes and in a method comparison study results showed good agreement with a previously published LC-MS/MS assay.Conclusions We have developed a simple method for measurement of plasma metanephrine, normetanephrine and 3-methoxytyramine using only 150 mu L of sample. There is minimal hands-on sample preparation required and the assay is suitable for routine use in a clinical laboratory.