Advanced magnetic resonance imaging of cerebral cavernous malformations: part II. Imaging of lesions in murine models.
Advanced magnetic resonance imaging of cerebral cavernous malformations: part II. Imaging of lesions in murine models.
复制标题
脑海绵状血管瘤的高级磁共振成像：第二部分。小鼠模型中病变的成像。
DOI：
10.1227/01.neu.0000315862.24920.49
复制
发表时间：
2008-10
期刊：
影响因子：
4.8
通讯作者：
Awad IA
中科院分区：
文献类型：
--
作者：
Shenkar R;Venkatasubramanian PN;Wyrwicz AM;Zhao JC;Shi C;Akers A;Marchuk DA;Awad IA
我们试图在小鼠Ccm 1和Ccm 2基因敲除模型中评估脑海绵状血管畸形（CCM）在磁共振（MR）成像上的表现，并建立一种可用于相关病理生物学研究的脑损伤定位技术在4.7 T和14.1 T条件下，对Ccm 1 +/-Trp 53-/-和Ccm 2 +/-Trp 53-/-组和28名对照组进行体内和/或体外梯度回波（T2*）加权MR成像。磁共振成像后，大脑被删除和染色的苏木精和伊红和细胞进行激光显微切割的分子生物学研究。T2* 加权磁共振成像的大脑在体内和离体显示病变类似于人类CCM突变小鼠，但不是在对照组动物。相关组织切片的立体定向定位和苏木精和伊红染色证实了病变组织学，并显示了同一大脑中的其他扩张毛细血管区域。14.1 T MR成像识别了一些病变，但4.7 T未识别。Ccm 1和β-肌动蛋白基因的PCR扩增从激光显微切割的病变和病变周围细胞提取的核酸中证明。高场MR成像技术为进一步研究体内疾病发病机制，以及病变的定位、分期和组织生物学解剖提供了新的机会，包括CCM病变发展的假定最早阶段。
We sought to assess the appearance of cerebral cavernous malformations (CCMs) on magnetic resonance (MR) imaging in murine Ccm1 and Ccm2 gene knockout models, and to develop a technique of lesion localization for correlative pathobiologic studies Brains from eighteen CCM mutant mice (Ccm1+/-Trp53-/- and Ccm2+/-Trp53-/-) and 28 controls were imaged by gradient recalled echo (T2*)-weighted MR at 4.7 T and 14.1 T in vivo and/or ex vivo. After MR imaging, the brains were removed and stained with hematoxylin and eosin and cells were laser microdissected for molecular biologic studies. T2*-weighted MR imaging of brains in vivo and ex vivo revealed lesions similar to human CCMs in mutant mice, but not in control animals. Stereotactic localization and hematoxylin and eosin-staining of correlative tissue sections confirmed lesion histology, and revealed other areas of dilated capillaries in the same brains. Some lesions were identified by MR imaging at 14.1 T, but not at 4.7 T. PCR amplification from Ccm1 and β-actin genes was demonstrated from nucleic acids extracted from laser microdissected lesional and perilesional cells. The high field MR imaging techniques offer new opportunities for further investigation of disease pathogenesis in vivo, and the localization, staging and histobiologic dissection of lesions, including the presumed earliest stages of CCM lesion development.