The miRNA machinery in primary cutaneous malignant melanoma, cutaneous malignant melanoma metastases and benign melanocytic nevi
The miRNA machinery in primary cutaneous malignant melanoma, cutaneous malignant melanoma metastases and benign melanocytic nevi
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DOI：
10.1007/s00441-012-1446-0
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发表时间：
2012-10-01
影响因子：
3.6
通讯作者：
Bechara, Falk G.
中科院分区：
文献类型：
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作者：
Sand, Michael;Skrygan, Marina;Bechara, Falk G.
虽然一些研究表明，在皮肤黑色素瘤的microRNA（miRNA）表达谱失调，一直很少有关于miRNA机制本身的研究。在这项研究中，我们调查了不同的miRNA机器组件在原发性皮肤恶性黑色素瘤（PCMM），皮肤恶性黑色素瘤转移（CMMM）和良性黑色素细胞痣（BMN）的mRNA表达谱。PCMM（n = 7）、CMMM（n = 6）和BMN（n = 7）患者均入选本研究。从肿瘤中心（病灶）和BMN（对照）采集打孔活检。与以往探索PCMM中特定miRNA簇的报道不同，本研究通过TaqMan实时逆转录聚合酶链反应（RT-PCR）检测Dicer、Drosha、Exp 5、DGCR 8和RISC组分PACT、argonaute-1、argonaute-2、TARBP 1、TARBP 2、MTDH和SND 1的mRNA表达水平。与PCMM相比，BMN中Argonaute-1、TARBP 2和SND 1的表达水平显着较高（p < 0.05）。与PCMM相比，CMMM中的TARBP 2表达水平显著更高（p < 0.05）。与PCMM和BMN相比，CMMM中SND 1表达水平显著更高（p < 0.05）。Dicer、Drosha、DGCR 8、Exp 5、argonaute-2、PACT、TARBP 1和MTDH表达水平在组内没有显示显著差异（p > 0.05）。这项研究的结果表明，与BMN相比，miRNA机器组件argonaute-1，TARBP 2和SND 1在PCMM和CMMM中失调，可能在恶性转化过程中发挥作用。
Although several studies have shown a dysregulation of microRNA (miRNA) expression profiles in cutaneous melanoma, there has been little research on the miRNA machinery itself. In this study, we investigated the mRNA expression profiles of different miRNA machinery components in primary cutaneous malignant melanoma (PCMM), cutaneous malignant melanoma metastases (CMMM) and benign melanocytic nevi (BMN). Patients with PCMM (n = 7), CMMM (n = 6) and BMN (n = 7) were included in the study. Punch biopsies were harvested from the centers of tumors (lesional) and from BMN (control). In contrast to previous reports exploring specific clusters of miRNAs in PCMM, the present study investigates mRNA expression levels of Dicer, Drosha, Exp5, DGCR8 and the RISC components PACT, argonaute-1, argonaute-2, TARBP1, TARBP2, MTDH and SND1, which were detected by TaqMan real-time reverse transcription polymerase chain reaction (RT-PCR). Argonaute-1, TARBP2 and SND1 expression levels were significantly higher in BMN compared to PCMM (p < 0.05). TARBP2 expression levels were significantly higher in CMMM compared to PCMM (p < 0.05). SND1 expression levels were significantly higher in CMMM compared to PCMM and BMN (p < 0.05). Dicer, Drosha, DGCR8, Exp5, argonaute-2, PACT, TARBP1 and MTDH expression levels showed no significant differences within groups (p > 0.05). The results of this study show that the miRNA machinery components argonaute-1, TARBP2 and SND1 are dysregulated in PCMM and CMMM compared to BMN and may play a role in the process of malignant transformation.