Fourier transform infrared-spectroscopic characterisation of isolated endodermal cell walls from plant roots: chemical nature in relation to anatomical development
Fourier transform infrared-spectroscopic characterisation of isolated endodermal cell walls from plant roots: chemical nature in relation to anatomical development
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DOI：
10.1007/s004250050758
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发表时间：
1999-10
期刊：
影响因子：
4.3
通讯作者：
J. Zeier;L. Schreiber
中科院分区：
文献类型：
--
作者：
J. Zeier;L. Schreiber
鹰嘴豆内胚层细胞壁的化学性质，君子兰和德国鸢尾（Iris germanica L.）用傅里叶变换红外光谱法（FTIR）研究。将观察到的频率分配给细胞壁中存在的官能团，并确定生物聚合物木栓素和木质素、细胞壁碳水化合物和蛋白质的相对量。红外吸收光谱表明三种不同的发育状态的孤立的内胚层细胞壁的结构特征：初级内皮层与凯氏带（状态I），次生内皮层木栓质lamellae（状态II），和三级内皮层与U形细胞壁沉积（状态III）。从这项研究中获得的数据进行比较，与以前的结果，通过化学降解分离的内胚层细胞壁和随后的测定单体降解产物的气相色谱和质谱。它的结论是，FTIR光谱是一种直接的和非破坏性的方法，适合于快速调查孤立的植物细胞壁。此外，观察到木栓素指定的吸收带消失后，与BF 3-甲醇的样品的酯交换证实，木栓素是完全降解，通过这种处理。
The chemical nature of enzymatically isolated endodermal cell walls fromCicer arietinumL.,Clivia miniataReg. andIris germanicaL. was studied by FTIR (Fourier transform infrared) spectroscopy. Observed frequencies were assigned to functional groups present in the cell wall and relative amounts of the biopolymers suberin and lignin, cell wall carbohydrates and proteins were determined. Infrared absorption spectra indicated structural characteristics for the three different developmental states of the isolated endodermal cell wall: primary endodermis with Casparian strips (state I), secondary endodermis with suberin lamellae (state II), and tertiary endodermis with U-shaped cell wall depositions (state III). The data obtained from this study are compared with previous results obtained by chemical degradation of isolated endodermal cell walls and subsequent determination of monomeric degradation products by gas chromatography and mass spectrometry. It is concluded that FTIR spectroscopy represents a direct and nondestructive method suitable for the rapid investigation of isolated plant cell walls. Furthermore, the observation that the suberin-assigned absorption bands disappeared after transesterification of the samples with BF3-methanol confirmed that suberin is completely degraded by this treatment.