Fumigant activity of volatiles of Streptomyces globisporus JK-1 against Penicillium italicum on Citrus microcarpa
Fumigant activity of volatiles of Streptomyces globisporus JK-1 against Penicillium italicum on Citrus microcarpa
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DOI：
10.1016/j.postharvbio.2010.06.003
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发表时间：
2010-11-01
影响因子：
7
通讯作者：
Hsiang, Tom
中科院分区：
文献类型：
--
作者：
Li, Qili;Ning, Ping;Hsiang, Tom
研究了球孢链霉菌JK-1菌株在小麦种子上生长的挥发性物质对意大利青霉的体外和植物体内抑菌活性。挥发物的存在抑制了真菌孢子萌发和菌丝生长，以及接种真菌的果实上的孢子形成和发病率。自然发病的果实，发病率从25%下降到7.5%。利用扫描电镜观察了意大利疫霉对沙糖蜜桔果实的侵染过程，发现意大利疫霉的挥发物对沙糖蜜桔孢子萌发有抑制作用，并使分生孢子梗和菌丝形态发生异常。采用气相色谱/质谱联用技术，从挥发物中鉴定出41种挥发性有机化合物。globisporus JK-1中，含量最高的化合物是反式-1，10-二甲基-反式-9-十烷醇（土臭素），这是一种土臭物质。其中，选择了8种工业级制剂进行进一步研究：苯乙醇、香紫苏烯、二甲基二硫醚、二甲基三硫醚、苯乙酮、D-柠檬烯、isoledene和aromadendrene。D-柠檬烯，isoledene和芳香树烯在体外和植物中在测试浓度下没有观察到的抗真菌活性。苯乙醇和香芹烯对沙糖桔的抑菌活性较弱，但对意大利疫霉的抑制作用不明显。二甲基二硫醚或二甲基三硫醚在100 μ L-1的处理容器中的空气空间的浓度下，在体外表现出抗真菌活性和有效的控制对沙塘柑。苯乙酮在100 μ L·L ~（-1）的浓度下表现出体外抑菌活性，在1000 μ L·L ~（-1）的最高浓度下对沙糖蜜柑有较好的防治效果。S.的挥发物。球孢子菌JK-1具有通过熏蒸作用控制柑橘蓝霉病的潜力。(C)2010爱思唯尔有限公司版权所有。
Antifungal activity against Penicillium italicum of volatile substances from Streptomyces globisporus JK-1 grown on autoclaved wheat seed was studied in vitro and in planta. Fungal spore germination and mycelial growth of P. italicum cultures as well as sporulation and disease incidence on fungal-inoculated fruit were suppressed in the presence of the volatiles. For naturally infected fruit, disease incidence was reduced from 25% to 7.5%. Suppression of the infection process of P. italicum on Shatang Mandarin fruit (Citrus microcarpa) was observed via scanning electronic microscopy, showing inhibited spore germination on the Shatang Mandarin, and abnormal morphology for conidiophores and hyphae exposed to the volatiles. Based on gas chromatography/mass spectrophotometric analyses, 41 volatile organic compounds were identified from the volatiles of S. globisporus JK-1, and the most abundant compound was trans-1,10-dimethyl-trans-9-decalol (geosmin), an earthy smelling substance. Among these, technical grade formulations of eight were chosen for further study: phenylethyl alcohol, caryophyllene, dimethyl disulfide, dimethyl trisulfide, acetophenone, D-limonene, isoledene, and aromadendrene. D-Limonene, isoledene and aromadendrene showed no observable antifungal activity in vitro and in planta at tested concentrations. Both phenylethyl alcohol and caryophyllene showed weak inhibitory activity in vitro but no significant efficacy against P. italicum on Shatang Mandarin. Dimethyl disulfide or dimethyl trisulfide showed antifungal activity in vitro and efficacious control on Shatang Mandarin at a concentration of 100 mu l L-1 of airspace in treatment containers. Acetophenone showed antifungal activity in vitro at a concentration of 100 mu L L-1 and efficacious control on Shatang Mandarin at the highest concentration of 1000 mu L L-1. Volatiles from S. globisporus JK-1 have potential for control of blue mold of citrus species through fumigant action. (C) 2010 Elsevier B.V. All rights reserved.