ULTRASTRUCTURE OF IMMATURE SERTOLI CELLS - MATURATION-LIKE CHANGES DURING CULTURE AND MAINTENANCE OF MITOTIC POTENTIALITY
ULTRASTRUCTURE OF IMMATURE SERTOLI CELLS - MATURATION-LIKE CHANGES DURING CULTURE AND MAINTENANCE OF MITOTIC POTENTIALITY
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DOI：
10.1095/biolreprod18.3.329
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发表时间：
1978-01-01
影响因子：
3.6
通讯作者：
FRITZ, IB
中科院分区：
文献类型：
--
作者：
SOLARI, AJ;FRITZ, IB
从10日龄大鼠睾丸分离的细胞聚集体在培养物中形成上皮集落。这些上皮细胞被确定为支持细胞（未成熟的支持细胞）的培养细胞的核和细胞质结构之间的密切相似性和支持细胞在体内。培养的支持细胞在含有促卵泡激素（FSH）的血清富集培养基中发生成熟样变化。这些变化包括染色质浓缩模式的变化，大核仁和核内折叠的发展，平滑的粗糙内质网和腔（顶端）分化的外观的进步优势。管腔表面的分化包括指状的细胞质延伸、微丝和微管的区域积累以及管腔附近相邻细胞之间的连接复合物。在FSH存在下，在培养的支持细胞群体中存在低但持续的有丝分裂活性。在培养物中从第4-15天观察到这种FSH依赖性有丝分裂活性。虽然支持细胞的几种结构分化在这些条件下在体外发展，这些细胞的管腔区域不分化的方式相同，在体内支持细胞的顶端区域。
Cell aggregates dissociated from 10 day old rat testes form epithelial colonies in culture. These epithelial cells were identified as supporting cells (immature Sertoli cells) by the close similarity between the nuclear and cytoplasmic structures of the cultured cells and those of supporting cells in vivo. The cultured supporting cells undergo maturation-like changes in serum-enriched media containing follicle stimulating hormone (FSH). These changes include a change in the pattern of chromatin condensation, the development of large nucleoli and nuclear infoldings, a progressive predominance of the smooth over the rough endoplasmic reticulum and the appearance of luminal (apical) differentiations. The differentiations of the luminal surface consist of finger-like cytoplasmic extensions, a regional accumulation of microfilaments and microtubules and the junctional complexes between neighboring cells near the lumen. In the presence of FSH there is a low but sustained mitotic activity in the population of cultured supporting cells. This FSH-dependent mitotic activity was observed from Day 4-15 in culture. While several structural differentiations of the supporting cells develop under these conditions in vitro, the luminal region of these cells does not differentiate in the same way as the apical region of Sertoli cells in vivo.