Effects of Norepinephrine on Immune Functions of Cultured Splenic Lymphocytes Exposed to Aluminum Trichloride
Effects of Norepinephrine on Immune Functions of Cultured Splenic Lymphocytes Exposed to Aluminum Trichloride
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去甲肾上腺素对三氯化铝作用下培养脾淋巴细胞免疫功能的影响
DOI：
10.1007/s12011-013-9729-1
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发表时间：
2013-06
影响因子：
3.9
通讯作者：
Yan-Fei Li
中科院分区：
文献类型：
--
作者：
Chong-Sheng Bai;Yan-Fei Han;Shi-Liang Xia;Yan-Fei Li
本研究旨在探讨去甲肾上腺素（NE）对铝离子（AlCl_3）染毒小鼠脾淋巴细胞的影响。本实验从体重约130 g的健康Wistar大鼠脾脏中分离淋巴细胞，并在含终浓度为0.552 mmol/L AlCl 3的RPMI-1640培养基中培养。将NE以终浓度0（对照组）、0.1（低剂量组）、1（中剂量组）和10（高剂量组）nmol/L加入培养的细胞中。不添加AlCl 3和NE作为空白（BG）。T淋巴细胞增殖;培养结束时检测IL-2、TNF-α、T淋巴细胞亚群、IgG、细胞内cAMP浓度和β2-肾上腺素能受体（β2-AR）密度。结果表明，NE可降低AlCl 3染毒小鼠T淋巴细胞增殖反应，降低IL-2、TNF-α含量，降低T淋巴细胞亚群，升高IgG含量，增加细胞内cAMP和β2-AR密度。与BG相比，AlCl 3暴露而不添加NE对这些指标的影响相似。结果提示NE通过β2-AR-cAMP信号通路加重了AlCl 3对淋巴细胞的免疫毒性，使淋巴细胞免疫功能紊乱。
The aim of this study was to investigate the effect of norepinephrine (NE) on spleen lymphocytes exposed to aluminum trichloride (AlCl3). In this experiment, lymphocytes were isolated from spleens of healthy Wistar rats weighing about 130 g and cultured with RPMI-1640 medium containing the final concentration of 0.552 mmol/L AlCl3. NE was added to the cultured cells at the final concentrations of 0 (control group), 0.1 (low-dose group), 1 (mid-dose group), and 10 (high-dose group) nmol/L. No addition of both AlCl3and NE serviced as blank (BG). The T lymphocyte proliferation; the contents of IL-2, TNF-α, and T lymphocyte subsets; immunoglobulin G (IgG) and intracellular cyclic adenosine monophosphate (cAMP) concentrations; and β2-adrenergic receptor (β2-AR) density were measured at the end of the culture. The result showed that NE decreased T lymphocyte proliferation and the contents of IL-2, TNF-α, and T lymphocyte subsets whereas increased the concentrations of IgG and intracellular cAMP and β2-AR density of the lymphocyte exposed to AlCl3. AlCl3exposure without adding NE showed the similar impacts on these measures compared with BG. The results suggested that NE aggravated AlCl3immunotoxicity on the lymphocytes and disordered the immune functions of the lymphocyte through the β2-AR-cAMP signal pathway.
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影响因子：
3.4
作者：
Bondy SC
通讯作者：
Bondy SC
影响因子：
100.3
作者：
Yvonne Bordon
通讯作者：
Yvonne Bordon
影响因子：
4.6
作者：
H. Besedovsky;E. Sorkin
通讯作者：
H. Besedovsky;E. Sorkin
影响因子：
3.3
作者：
Y. Qiu;Chun Cheng;L. Dai;Yu-ping Peng
通讯作者：
Y. Qiu;Chun Cheng;L. Dai;Yu-ping Peng
影响因子：
6.1
作者：
Heijink, IH;van den Berge, M;Kauffman, HF
通讯作者：
Kauffman, HF