The effect of seasons on Brazilian red propolis and its botanical source: chemical composition and antibacterial activity
The effect of seasons on Brazilian red propolis and its botanical source: chemical composition and antibacterial activity
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DOI：
10.1080/14786419.2016.1239088
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发表时间：
2017-01-01
影响因子：
2.2
通讯作者：
Rosalen, Pedro L.
中科院分区：
文献类型：
--
作者：
Bueno-Silva, Bruno;Marsola, Alexandre;Rosalen, Pedro L.
本研究旨在评价不同季节对巴西红蜂胶及其植物来源的化学成分和抗菌活性的影响。BRP是在一年的时间里从巴西东北部的阿拉戈斯州的Maceio收集的。通过理化分析和高效液相测定化学成分，通过测定最小抑菌浓度和最小杀菌浓度(MIC和MBC)来评价其对变形链球菌、远缘链球菌、金黄色葡萄球菌和奈氏放线菌的抑菌活性。比较化学图谱因采集期不同而不同。芦荟素是蜂胶和树脂中含量最丰富的化合物，异甘草素、(3S)-新香草醇、(3S)-香草醇是巴西红蜂胶抑菌活性的主要成分。MIC在15.6~125g/m L，MBC在31.2~500g/m L。因此，采集蜂胶及其植物来源的季节确实会影响其化学成分，导致其抗菌活性的变化。
The aim of this study was to evaluate the effect of seasons on the chemical composition and antibacterial activity of Brazilian red propolis (BRP) and its plant source. BRP was collected from Maceio, Alagoas state, north-east of Brazil, during one year. Chemical composition was determined by physicochemical analyses and HPLC while antimicrobial activity was assessed against Streptococcus mutans, Streptococcus sobrinus, Staphylococcus aureus and Actinomyces naeslundii by determining the minimal inhibitory and bactericidal concentrations (MIC and MBC, respectively). The comparative chemical profiles varied quantitatively according to the collection period. Formononetin was the most abundant compound in both propolis and resin, while isoliquiritigenin, (3S)-neovestitol, (3S)-vestitol are suggested to be responsible for antimicrobial activity of Brazilian red propolis. MIC varied from 15.6 to 125g/mL, whereas MBC varied from 31.2 to 500g/mL. Therefore, season in which propolis and its botanical source are collected indeed influences their chemical compositions, resulting in variations in their antibacterial activity.[GRAPHICS]