Using subcritical/supercritical fluid chromatography to separate acidic, basic, and neutral compounds over an ionic liquid-functionalized stationary phase
Using subcritical/supercritical fluid chromatography to separate acidic, basic, and neutral compounds over an ionic liquid-functionalized stationary phase
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DOI：
10.1016/j.chroma.2009.02.057
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发表时间：
2009-04-17
影响因子：
4.1
通讯作者：
Wei, Guor-Tzo
中科院分区：
文献类型：
--
作者：
Chou, Fu-Min;Wang, Wei-Ting;Wei, Guor-Tzo
我们将离子液体（IL）功能化的固定相-基于1-辛基-3-丙基咪唑氯化物共价键合到硅胶上-填充到3.2 mm x 250 mm柱中，用于使用二氧化碳亚临界/超临界流体色谱（SFC）同时分离酸性、碱性和中性化合物，并考察了压力、温度、共溶剂、和添加剂对分析物保留行为的影响。用于SFC分离的测试的模型化合物是对乙酰氨基酚、美托洛尔、非诺洛芬、布洛芬、萘和睾酮。结果表明，离子液体修饰固定相与待测物之间的氢键和疏水相互作用参与了分离过程。通过SFC同时分离酸性、碱性和中性化合物在共溶剂含量为20%MeOH、压力为110巴和柱温为35 ℃时是成功的。保留时间和50 ppm峰面积的相对标准偏差均小于4%和8%（n = 6）。(C)2009爱思唯尔有限公司版权所有。
We packed an ionic liquid (IL)-functionalized stationary phase - based on 1-octyl-3-propylimidazolium chloride covalently bounded to silica gel - into a 3.2 mm x 250 mm column for the simultaneous separation of acidic, basic, and neutral compounds using carbon dioxide subcritical/supercritical fluid chromatography (SFC), and examined the effects of the pressure, temperature, co-solvents, and additives on the retention behavior of the analytes. The model compounds tested for SFC separation are acetaminophen, metoprolol, fenoprofen, ibuprofen, naphthalene, and testosterone. The data indicate that hydrogen-bonding and hydrophobic interactions between the analytes and the IL-modified stationary phase seem to involve in the separation process. Simultaneous separation of acidic, basic, and neutral compounds via SFC was successful at a co-solvent content of 20% MeOH, a pressure of 110bar, and a column temperature of 35 C. The relative standard deviations of the retention times and peak areas at 50 ppm were all less than 4 and 8% (n = 6), respectively. (C) 2009 Elsevier B.V. All rights reserved.