Amidolytic, procoagulant, and activation-suppressing proteins produced by contact activation of blood factor XII in buffer solution.
Amidolytic, procoagulant, and activation-suppressing proteins produced by contact activation of blood factor XII in buffer solution.
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DOI：
10.1016/j.biomaterials.2011.09.020
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发表时间：
2011-12
期刊：
影响因子：
14
通讯作者：
Vogler, Erwin A.
中科院分区：
文献类型：
--
作者：
Golas, Avantika;Yeh, Chyi-Huey Joshua;Siedlecki, Christopher A.;Vogler, Erwin A.
通过在缓冲液中接触激活血酶原因子XII(FXII，Hageman因子)而产生的具有酰胺分解或促凝血活性的酶的相对比例取决于激活剂的表面化学/能量。因此，发色法测氨解活性(S-2302色原中氨基酸键的断裂)与传统的血浆凝血时间测定法(引起血浆凝血的蛋白酶活性)不相关。酰胺化活性与活化剂-颗粒表面能无显著差异，这里测量的是水粘着张力；其中是纯缓冲液界面张力，θa是推进接触角。相比之下，促凝血剂的活性变化为τo的抛物线函数，在激活剂表面能的疏水和亲水两端都很高，并在20<；τo<；40 MJ/m2(55o；θa<；75O)范围内降至广泛的最小值，证实和扩展了先前发表的工作。从这些功能分析中推测，FXII(又名：FXII)的接触激活产生了数量未知的蛋白质片段。自动激活)，而不是普遍认为的αFXIIa和βFXIIa。由20<；τo<；40 MJ/m2(55o；θa<；75O)表面能范围内的激活剂颗粒产生的自活化产物通过未知的生物物理化学抑制从疏水或亲水表面能极端值中选择的激活剂产生促凝血酶。抑制蛋白可能是实验观察到的自激活反应的自动抑制的原因。
The relative proportions of enzymes with amidolytic or procoagulant activity produced by contact activation of the blood zymogen factor XII (FXII, Hageman factor) in buffer solution depends on activator surface chemistry/energy. As a consequence, chromogenic assay of amidolytic activity (cleavage of amino acid bonds in s-2302 chromogen) does not correlate with the traditional plasma-coagulation-time assay for procoagulant activity (protease activity inducing clotting of blood plasma). Amidolytic activity did not vary significantly with activator-particle surface energy, herein measured as water adhesion tension ; where is pure buffer interfacial tension and θa is the advancing contact angle. By contrast, procoagulant activity varied as a parabolic-like function of τo, high at both hydrophobic and hydrophilic extremes of activator surface energy and falling through a broad minimum within a 20 <τo < 40 mJ/m2 ( 55o <θa < 75o) range, corroborating and expanding previously-published work. It is inferred from these functional assays that an unknown number of protein fragments are produced by contact activation of FXII (a.k.a. autoactivation) rather than just αFXIIa and βFXIIa as popularly believed. Autoactivation products produced by activator particles within the 20 <τo < 40 mJ/m2 (55o <θa < 75o) surface-energy range suppresses production of procoagulant enzymes by activators selected from the hydrophobic or hydrophilic surface-energy extremes through as-yet unknown biophysical chemistry. Suppression proteins may be responsible for the experimentally-observed autoinhibition of the autoactivation reaction.
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影响因子：
14
作者：
Noh, Hyeran;Vogler, Erwin A.
通讯作者：
Vogler, Erwin A.
DOI：
10.1084/jem.147.3.719
发表时间：
1978-03-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
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通讯作者：
Griffin JH
影响因子：
3.9
作者：
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通讯作者：
COLMAN, RW
影响因子：
7.5
作者：
Alesci, Sonja;Borggrefe, Martin;Dempfle, Carl-Erik
通讯作者：
Dempfle, Carl-Erik
影响因子：
4.3
作者：
Chen, Xiaoyun;Wang, Jie;Chen, Zhan
通讯作者：
Chen, Zhan