Glutamate-induced Production of Nitric Oxide in Guinea Pig Vestibular Sensory Cells
Glutamate-induced Production of Nitric Oxide in Guinea Pig Vestibular Sensory Cells
复制标题
谷氨酸诱导豚鼠前庭感觉细胞产生一氧化氮
作者：
M. Anniko
用一氧化氮（NO）荧光指示剂<$A-2DA直接检测谷氨酸（Glu）诱导的豚鼠前庭器官NO的产生，并对豚鼠椭圆囊斑和前庭感觉细胞的NO产生部位进行了观察。用谷氨酸、NMDA和AMPA刺激感觉细胞，其荧光强度增强。这是第一个直接的证据表明，一氧化氮生产的前庭终末器官。NO可能在谷氨酸诱发的耳毒性中起重要作用，并参与内耳疾病的发生。
Glutamate-induced production of nitric oxide (NO) in the vestibular organ of the guinea pig was investigated using the new fluorescence indicator, DAF-2DA, for direct detection of NO. Utricular maculae and isolated vestibular sensory cells were examined to locate NO production sites. The fluorescence intensity of the sensory cells was augmented by stimulation with glutamate, NMDA and AMPA. This is the first direct evidence of NO production in the vestibular end organs. NO may play an important role in the glutamate-induced ototoxicity and also be involved in disease of the inner ear.
DOI：
10.1073/pnas.88.14.6368
发表时间：
1991-07-01
影响因子：
11.1
作者：
DAWSON, VL;DAWSON, TM;SNYDER, SH
通讯作者：
SNYDER, SH
一氧化氮、超氧化物和过氧亚硝酸盐：好的、坏的和丑陋的。
DOI：
10.1152/ajpcell.1996.271.5.c1424
发表时间：
1996
期刊：
The American journal of physiology
影响因子：
--
作者：
Beckman,JS;Koppenol,WH
通讯作者：
Koppenol,WH