Marking multi-channel silicon-substrate electrode recording sites using radiofrequency lesions
Marking multi-channel silicon-substrate electrode recording sites using radiofrequency lesions
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DOI：
10.1016/j.jneumeth.2005.06.012
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发表时间：
2006-01-30
影响因子：
3
通讯作者：
Bauer, CA
中科院分区：
文献类型：
--
作者：
Brozoski, TJ;Caspary, DM;Bauer, CA
硅衬底多通道电极(多探针)已被证明在各种电生理任务中有用。当使用多探头时，识别每个通道的位置通常是有用的，例如，当记录来自不同结构的单个单元活动时。电极部位的损伤标记已被使用多年。电解或直流(DC)损伤已成功地用于标记大鼠海马区的多探针位置[TownsenG，Peloquin P，Krousterman F，Hetke JF，Leung LS]。使用硅多通道电极进行记录和标记。Brain Res Brain ResProtoc 2002；9：122-9]。本方法使用射频(RF)损伤来清楚地标记16通道线阵多探头在栗鼠下丘的16个记录点。使用商用射频损伤器作为电流源，与适应多探头配置的定制连接器相结合。体外台架测试被用来建立电流-电压-时间参数，以及检查多探头的完整性和射频性能。在活体应用中，从急性实验对象获得的33组耳丘连续切片(即探头轨迹)中，有21组清晰地显示了单个通道多探头的记录位置，即损伤后最长存活时间为2小时。RF方法的优点包括良好的体外校准方法以及对探头完整性的低影响。标记单个频道站点的RF方法在各种应用中应该是有用的。(C)2005 Elsevier B.V.保留所有权利。
Silicon-substrate multi-channel electrodes (multiprobes) have proven useful in a variety of electrophysiological tasks. When using multiprobes it is often useful to identify the site of each channel, e.g., when recording single-unit activity from a heterogeneous structure. Lesion marking of electrode sites has been used for many years. Electrolytic, or direct current (DC) lesions, have been used successfully to mark multiprobe sites in rat hippocampus [Townsend G, Peloquin P, Kloosterman F, Hetke JF, Leung LS. Recording and marking with silicon multichannel electrodes. Brain Res Brain Res Protoc 2002;9:122-9]. The present method used radio-frequency (rf) lesions to distinctly mark each of the 16 recording sites of 16-channel linear array multiprobes, in chinchilla inferior colliculus. A commercial radio-frequency lesioner was used as the current source, in conjunction with custom connectors adapted to the multiprobe configuration. In vitro bench testing was used to establish current-voltage-time parameters, as well as to check multiprobe integrity and radio-frequency performance. In in vivo application, visualization of individual-channel multiprobe recording sites was clear in 21 out of 33 sets of collicular serial-sections (i.e., probe tracks) obtained from acute experimental subjects, i.e., maximum post-lesion survival time of 2 h. Advantages of the rf method include well-documented methods of in vitro calibration as well as low impact on probe integrity. The rf method of marking individual-channel sites should be useful in a variety of applications. (c) 2005 Elsevier B.V. All rights reserved.