Functional Genomic Analysis of the SPL9 Gene in Arabidopsis thaliana under Low Phosphate Conditions
Functional Genomic Analysis of the SPL9 Gene in Arabidopsis thaliana under Low Phosphate Conditions
复制标题
低磷条件下拟南芥SPL9基因的功能基因组分析
DOI：
10.1134/s1021443722020091
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发表时间：
2022-03
影响因子：
1.4
通讯作者：
Huan Dong
中科院分区：
文献类型：
--
作者：
Kaijian Lei;Huan Dong
摘要无机磷（Pi）是一种重要的营养元素，是植物生长的限制因子。据报道，SPL家族成员，如SPL 3，通过控制Pi缺乏反应基因的表达来调节Pi缺乏反应。为了阐明SPL 9是否响应低磷胁迫，我们在正常和低磷条件下研究了转基因拟南芥中过表达rSPL 9（R9）的表型并进行了RNA测序分析。与野生型相比，R9在缺磷条件下地上部花色素苷积累减少，而磷含量增加。通过RNA-seq分析，在R9植株中检测到217个基因在磷充足条件下显著差异表达，在磷缺乏条件下差异表达的基因为121个。在缺磷条件下，MYB 62和ZAT 6是两个重要的差异表达基因，它们都参与了磷的吸收过程。此外，这些DEG还包括多种蛋白激酶、茉莉酸响应基因和盐胁迫相关基因。与水解酶和转移酶活性相关的基因也受到Pi缺乏的差异调节，如细胞色素P450单加氧酶。特别值得注意的是，转录因子AP 2-EREBP和bHLH家族的成员是在Pi充足和Pi缺乏条件下鉴定的最显著差异调节的基因之一。总之，我们对R9转录组的分析突出了SPL 9在Pi缺乏条件下的重要性。除胁迫和防御反应基因外，R9转录组还表征了缺磷下乙烯或茉莉酸信号的诱导。
Abstract Inorganic phosphate (Pi) is an essential nutrient, which is often served as a limiting factor in plant growth. It has been reported that SPL family members, such as SPL3, regulate Pi deficiency responses by controlling the expression of Pi deficiency responsive genes. To elucidate whether SPL9 respond to low phosphorus stress, we investigated the phenotypes and conducted RNA sequencing analysis in transgenic Arabidopsis thaliana with overexpressing rSPL9 (R9) under conditions of both normal and low Pi availability. Compared with wild-type plants, R9 showed decreased anthocyanin accumulation and increased Pi contents in shoots under Pi deficiency. Through RNA-seq analysis compared with wild-type plants, we detected 217 genes significantly differentially expressed in conditions of Pi sufficiency, and 121 genes differentially expressed in conditions of Pi deficiency in R9 plants. Under Pi deficiency, MYB62 and ZAT6 are two important differentially expressed genes (DEGs) that both regulate the Pi uptake processes. In addition, these DEGs included multiple protein kinases, jasmonic acid response genes and genes related to salt stress responses. Genes associated with hydrolase and transferase activity were also differentially regulated by Pi deficiency, such as cytochrome P450 monooxygenases. Of particular note, the transcription factor AP2-EREBP and members of the bHLH family were among the most significantly differentially regulated genes identified under both Pi sufficient and Pi deficient conditions. In conclusion, our analysis of the R9 transcriptome highlights the importance of SPL9 under conditions of Pi-deficiency. Except for stress and defense response genes, the R9 transcriptome also characterized the induction of ethylene or jasmonic acid signaling under Pi deficiency.
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影响因子：
4.5
作者：
He J;Xu M;Willmann MR;McCormick K;Hu T;Yang L;Starker CG;Voytas DF;Meyers BC;Poethig RS
通讯作者：
Poethig RS
DOI：
10.1073/pnas.1009975107
发表时间：
2010-12-07
影响因子：
11.1
作者：
Lee, Sungbeom;Badieyan, Somayesadat;Tholl, Dorothea
通讯作者：
Tholl, Dorothea
影响因子：
4.4
作者：
Lan P;Li W;Schmidt W
通讯作者：
Schmidt W
影响因子：
11.6
作者：
Guo, Changkui;Xu, Yunmin;Wu, Gang
通讯作者：
Wu, Gang
DOI：
10.1016/j.bbrc.2014.02.013
发表时间：
2014-03-07
影响因子：
3.1
作者：
Idanheimo, Niina;Gauthier, Adrien;Wrzaczek, Michael
通讯作者：
Wrzaczek, Michael