In vitro cytotoxicity and dentin permeability of HEMA
In vitro cytotoxicity and dentin permeability of HEMA
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DOI：
10.1016/s0099-2399(06)80141-x
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发表时间：
1996-05-01
影响因子：
4.2
通讯作者：
Holz, J
中科院分区：
文献类型：
--
作者：
Bouillaguet, S;Wataha, JC;Holz, J
采用体外扩散室法测定2-羟乙基甲基丙烯酸酯（HEMA）在牙本质蚀刻盘中的扩散。采用紫外光谱法测定HEMA在牙本质中扩散的浓度，并考察初始浓度、牙本质厚度和背压对扩散的影响。用BALB/c 3T3小鼠成纤维细胞与HEMA直接接触12或24小时来测定HEMA的细胞毒性。在所有条件下，HEMA都能迅速扩散通过牙本质，但增加厚度、背压或降低初始浓度都会降低扩散。HEMA的渗透系数约为0.0003 cm/min，通过0.5 mm牙本质的扩散使HEMA浓度降低了约6000倍（H2O背压为10 cm）。结论：牙本质对HEMA产生急性细胞毒性的风险可能较低，但牙本质厚度降低、缺乏聚合或暴露时间延长可能显著增加风险。
An in vitro diffusion chamber was used to measure the diffusion of 2-hydroxyethyl methacrylate (HEMA) through etched human dentin disks. Concentrations of HEMA, which diffused through dentin, were measured by ultraviolet spectroscopy, and the effect of initial HEMA concentration, dentin thickness, and back pressure on diffusion were assessed. The cytotoxicity of HEMA was determined using BALB/c 3T3 mouse fibroblasts in direct contact with HEMA for 12 or 24 h. HEMA diffused rapidly through dentin under all conditions, but increased thickness, back pressure, or decreased initial concentration all reduced diffusion. The permeability coefficient of HEMA was similar to 0.0003 cm/min, and diffusion through 0.5 mm of dentin reduced the HEMA concentration by a factor of similar to 6,000 (with 10 cm of H2O back pressure). It was concluded that the risk of acute cytotoxicity to HEMA through dentin was probably low, but that decreased dentin thickness, lack of polymerization, or extended exposure times might increase the risk significantly.