CRISPR/Cas9-Mediated Genome Editing in Zebrafish.

CRISPR/Cas9-Mediated Genome Editing in Zebrafish.
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CRISPR/Cas9 介导的斑马鱼基因组编辑。

DOI:
10.1007/978-1-0716-2990-1_17
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发表时间:
2023
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
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通讯作者:
Shavit,JordanA
Shavit,JordanA
中科院分区:
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文献类型:
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作者:
Daniel,JeffreyG;Yu,Xinge;Ferguson,AllisonC;Shavit,JordanA

文献摘要

相似文献

CRISPR/Cas9系统是斑马鱼基因组编辑的强大工具。该工作流程利用了斑马鱼的遗传易处理性,并将允许用户编辑基因组位点并使用选择性育种产生突变系。然后,研究人员可以利用建立的品系进行下游遗传和表型分析。
The CRISPR/Cas9 system is a powerful tool for genome editing in zebrafish. This workflow takes advantage of the genetic tractability of zebrafish and will allow users to edit genomic sites and produce mutant lines using selective breeding. Established lines may then be employed by researchers for downstream genetic and phenotypic analyses.