The interaction of styrene maleic acid copolymers with phospholipids in Langmuir monolayers, vesicles and nanodiscs; a structural study.
The interaction of styrene maleic acid copolymers with phospholipids in Langmuir monolayers, vesicles and nanodiscs; a structural study.
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DOI：
10.1016/j.jcis.2022.03.102
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发表时间：
2022-03
影响因子：
9.9
通讯作者：
Stephen C. L. Hall;Cecilia Tognoloni;R. Campbell;J. Richens;P. O'shea;A. Terry;G. Price;T. Dafforn;K. Edler;T. Arnold
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文献类型：
--
作者：
Stephen C. L. Hall;Cecilia Tognoloni;R. Campbell;J. Richens;P. O'shea;A. Terry;G. Price;T. Dafforn;K. Edler;T. Arnold
假设两亲性苯乙烯马来酸共聚物与磷脂在水溶液中的自组装导致形成含有由聚合物带包封的磷脂双层的平面段的“纳米盘”。最近，研究报告了脂质在溶液中的纳米盘和脂质的外部来源之间快速交换。关于聚合物-脂质相互作用的细节、影响脂质交换的因素和这种交换过程的结构效应，仍然存在悬而未决的问题。在这里，纳米盘的动态行为进行了研究，特别是膜电荷和聚合物chemistry.ExperimentsTwo模型系统的作用进行了研究：荧光标记的磷脂囊泡，和Langmuir单层磷脂。使用荧光光谱和时间分辨中子反射仪，膜电位，单层结构和组合物的监测相对于聚合物和nanodisces interactions.FindingsIn外部脂质的存在下，聚合物链嵌入整个脂质膜，其程度取决于净膜电荷的时间。由三种不同聚合物稳定的纳米盘将全部与单层交换脂质和聚合物至不同程度，这与稳定聚合物带的性质有关。这些结果证明了纳米盘的动态性质，其与局部环境相互作用，并且可能在使用的所有阶段存款脂质和聚合物。
HypothesisSelf-assembly of amphipathic styrene maleic acid copolymers with phospholipids in aqueous solution results in the formation of ‘nanodiscs’ containing a planar segment of phospholipid bilayer encapsulated by a polymer belt. Recently, studies have reported that lipids rapidly exchange between both nanodiscs in solution and external sources of lipids. Outstanding questions remain regarding details of polymer-lipid interactions, factors influencing lipid exchange and structural effects of such exchange processes. Here, the dynamic behaviour of nanodiscs is investigated, specifically the role of membrane charge and polymer chemistry.ExperimentsTwo model systems are investigated: fluorescently labelled phospholipid vesicles, and Langmuir monolayers of phospholipids. Using fluorescence spectroscopy and time-resolved neutron reflectometry, the membrane potential, monolayer structure and composition are monitored with respect to time upon polymer and nanodisc interactions.FindingsIn the presence of external lipids, polymer chains embed throughout lipid membranes, the extent of which is governed by the net membrane charge. Nanodiscs stabilised by three different polymers will all exchange lipids and polymer with monolayers to differing extents, related to the properties of the stabilising polymer belt. These results demonstrate the dynamic nature of nanodiscs which interact with the local environment and are likely to deposit both lipids and polymer at all stages of use.