A study on simultaneous photolimitation and photoinhibition in dense microalgal cultures taking into account incident and averaged irradiances
A study on simultaneous photolimitation and photoinhibition in dense microalgal cultures taking into account incident and averaged irradiances
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DOI：
10.1016/0168-1656(95)00144-1
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发表时间：
1996-02-12
影响因子：
4.1
通讯作者：
Camacho, FG
中科院分区：
文献类型：
--
作者：
Grima, EM;Sevilla, JMF;Camacho, FG
从海洋微藻等鞭金藻 (CCAP 927/15) 的恒化培养物中观察到同时的光限制和光抑制。每种现象的程度取决于培养物内部的光梯度，因此也取决于入射辐照度。研究了三种入射辐照度 I-o 下培养物内部生物量浓度和平均辐照度随稀释率的变化（从 I-o = 820 到 3270 mu E m(-2) S-1）。在 I-o 高于 1630 mu E m(-2) s(-1) 时，观察到光抑制效应，尽管无论入射辐照度如何，比生长速率仍然是平均辐照度的双曲函数。为了计算平均辐照度，提出了一种圆柱几何形状的三维辐照度分布模型，由于考虑了沿垂直轴的光照变化，因此相对于迄今为止使用的其他方法改进了培养物内部辐照度场的估计。最后，考虑到比生长速率与平均辐照度相关，并且代表细胞对光适应性的参数是细胞暴露的最大辐照度（即入射辐照度）的函数，提出了一种同时模拟光限制和光抑制的新方法。
From chemostat cultures of the marine microalga Isochrysis galbana (CCAP 927/15) simultaneous photolimitation and photoinhibition was observed. The extent of each phenomenon depends on the light gradient inside the culture and therefore on the incident irradiance. Variations in biomass concentration and average irradiance inside the culture with dilution rate at three incident irradiances, I-o, were studied (from I-o = 820 to 3270 mu E m(-2) S-1). At I-o above 1630 mu E m(-2) s(-1) a photoinhibition effect was observed, although the specific growth rate remained a hyperbolic function of average irradiance regardless of incident irradiance. To calculate average irradiance, a three-dimensional irradiance distribution model for cylindrical geometry is proposed, improving the estimation of the irradiance field inside culture with regard to other methods used up to now since the variations in illumination along the vertical axis are considered. Lastly, a new approach to model simultaneous photolimitation and photoinhibition is proposed by considering that specific growth rate is related to average irradiance and that parameters representing the cell adaptability to light are a function of the maximum irradiation at which cells are exposed, that is, the incident irradiance.