FUNCTIONAL CONSERVATION OF THE SECRETION AND TRANSLOCATION MACHINERY FOR VIRULENCE PROTEINS OF YERSINIAE, SALMONELLAE AND SHIGELLAE
FUNCTIONAL CONSERVATION OF THE SECRETION AND TRANSLOCATION MACHINERY FOR VIRULENCE PROTEINS OF YERSINIAE, SALMONELLAE AND SHIGELLAE
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DOI：
10.1002/j.1460-2075.1995.tb00092.x
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发表时间：
1995-09-01
期刊：
影响因子：
11.4
通讯作者：
WOLFWATZ, H
中科院分区：
文献类型：
--
作者：
ROSQVIST, R;HAKANSSON, S;WOLFWATZ, H
耶尔森菌属、志贺氏菌属和沙门氏菌属的毒力细菌分别通过III型分泌途径分泌YOPs、iPAs和Sips等毒力决定基因。福氏志贺菌的IPAB蛋白在假结核耶尔森菌中表达，并在相同的条件下分泌YopE。同样，YopE由鼠伤寒沙门氏菌野生型菌株LT2分泌，但YopE不是由同基因的不同突变株分泌的，IPAB和YopE的分泌都需要它们各自的伴侣IpgC和YopE。此外，含有YopE的鼠伤寒沙门氏菌表达YopE介导的细胞毒性。在感染的HeLa细胞胞浆中检测到YopE，且YopE转位的量与细胞毒性的程度有关，庆大霉素的加入可以阻止YopE的易位和细胞毒性。在感染前用细胞松弛素D处理HeLa细胞阻止了细菌的内化，但仍观察到YopE的易位。这些结果支持YopE是由表面定位的细菌通过质膜转运的假设。我们提出，毒力沙门氏菌和志贺氏菌通过与耶尔森菌类似的功能保守的分泌/易位机制将毒力效应分子递送到靶细胞。
Virulent bacteria of the genera Yersinia, Shigella and Salmonella secrete a number of virulence determinants, Yops, Ipas and Sips respectively, by a type III secretion pathway, The IpaB protein of Shigella flexneri was expressed in Yersinia pseudotuberculosis and found to be secreted under the same conditions required for Yop secretion, Likewise, YopE was secreted by the wild-type strain LT2 of Salmonella typhimurium, but YopE was not secreted by the isogenic invA mutant, Secretion of both IpaB and YopE required their respective chaperones, IpgC and YerA., In addition, yopE-containing S.typhimurium expressed a YopE-mediated cytotoxicity on cultured HeLa cells. YopE was detected in the cytosol of the infected HeLa cells and the amount of translocated YopE correlated with the degree of cytotoxicity, Both translocation and cytotoxicity were prevented by the addition of gentamicin. Treatment of HeLa cells with cytochalasin D prior to infection prevented internalization of bacteria, but translocation of YopE was still observed, These results favour the hypothesis that YopE is translocated through the plasma membrane by surface-located bacteria, We propose that virulent Salmonella and Shigella deliver virulence effector molecules into the target cell through the utilization of a functionally conserved secretion/translocation machinery similar to that shown for Yersinia.