Grass carp prolactin: Molecular cloning, tissue expression, intrapituitary autoregulation by prolactin and paracrine regulation by growth hormone and luteinizing hormone
Grass carp prolactin: Molecular cloning, tissue expression, intrapituitary autoregulation by prolactin and paracrine regulation by growth hormone and luteinizing hormone
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DOI：
10.1016/j.mce.2014.10.010
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发表时间：
2015-01-05
影响因子：
4.1
通讯作者：
Wong, Anderson O. L.
中科院分区：
文献类型：
--
作者：
Lin, Chengyuan;Jiang, Xue;Wong, Anderson O. L.
催乳素（PRL）是一种具有多种功能的垂体激素，它受到下丘脑和外周信号的控制。通过自分泌/旁分泌机制调节PRL的头内表达也有报道，但在低等脊椎动物中仍然缺乏类似的信息。为了阐明PRL在鱼类模型中的自分泌/旁分泌调控作用，我们克隆了草鱼PRL，并证实了其在草鱼垂体中的表达。草鱼垂体细胞局部分泌PRL可抑制PRL的释放，同时PRL的产生和mRNA水平升高。生长激素（GH）刺激旁分泌可上调PRL分泌、PRL生成和PRL转录物表达，而促黄体生成素（LH）的局部作用则相反。显然，PRL、GH和LH通过自分泌/旁分泌机制的局部相互作用，通过对PRL mRNA稳定性和基因转录的差异调控，改变了鲤鱼垂体细胞中PRL的产生。2014爱思唯尔爱尔兰有限公司版权所有。
Prolactin (PRL), a pituitary hormone with diverse functions, is well-documented to be under the control of both hypothalamic and peripheral signals. Intrapituitary modulation of PRL expression via autocrine/paracrine mechanisms has also been reported, but similar information is still lacking in lower vertebrates. To shed light on autocrine/paracrine regulation of PRL in fish model, grass carp PRL was cloned and its expression in the carp pituitary has been confirmed. In grass carp pituitary cells, local secretion of PRL could suppress PRL release with concurrent rises in PRL production and mRNA levels. Paracrine stimulation by growth hormone (GH) was found to up- regulate PRL secretion, PRL production and PRL transcript expression, whereas the opposite was true for the local actions of luteinizing hormone (LH). Apparently, local interactions of PRL, GH and LH via autocrine/paracrine mechanisms could modify PRL production in carp pituitary cells through differential regulation of PRL mRNA stability and gene transcription. (C) 2014 Elsevier Ireland Ltd. All rights reserved.