Long non-coding RNA XLOC_008466 acts as an oncogenic molecular in cervical cancer tumorigenesis
Long non-coding RNA XLOC_008466 acts as an oncogenic molecular in cervical cancer tumorigenesis
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DOI：
10.1016/j.biopha.2017.11.143
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发表时间：
2018-02-01
影响因子：
7.5
通讯作者：
Chen, Yu-Mei
中科院分区：
文献类型：
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作者：
Guo, Fang;Chen, Yun-Zhi;Chen, Yu-Mei
宫颈癌是女性生殖系统最常见的恶性肿瘤之一。长链非编码rna （lncRNAs）已被证实参与宫颈癌的发生。在本研究中，我们探讨lncRNA XLOC_008466在宫颈癌发生发展中的作用。结果显示，XLOC_008466在宫颈癌组织和细胞中的表达水平较正常对照上调。体外功能实验、CCK-8实验和集落形成实验表明，敲除XLOC_008466可抑制宫颈癌细胞的增殖。流式细胞术和transwell实验显示，敲低XLOC_008466可诱导G0/G1期阻滞，加重细胞凋亡。在体内，敲低XLOC_008466抑制肿瘤生长。生物信息学分析显示，XLOC_008466将miR-216b与3'-UTR上的互补结合位点海绵化。总之，我们的研究揭示了XLOC_008466在宫颈癌发生中的促瘤作用，为宫颈癌提供了新的分子机制和治疗靶点。
Cervical cancer is one of the most common malignant carcinomas in the female reproductive system. Long non-coding RNAs (lncRNAs) have been verified to participate in the tumorigenesis of cervical cancer. In present study, we investigate the role of lncRNA XLOC_008466 in the occurrence and progression of cervical cancer. Results showed that XLOC_008466 expression was up-regulated in cervical cancer tissue and cells compared to normal controls. In vitro functional experiments, CCK-8 assay and colony formation assay showed that XLOC_008466 knockdown suppressed the proliferation of cervical cancer cells. Flow cytometry and transwell assay showed that XLOC_008466 knockdown induced G0/G1 phase arrest and aggravated the apoptosis. In vivo, XLOC_008466 knockdown inhibited the tumor growth. Bioinformatics analysis revealed that XLOC_008466 sponged miR-216b with the complementary binding sites at 3'-UTR. Overall, our study reveals the tumor promoting role of XLOC_008466 in cervical cancer carcinogenesis, providing a novel molecular mechanism and therapeutic target for cervical cancer.