REMOVAL OF REPEATED SEQUENCES FROM HYBRIDIZATION PROBES
REMOVAL OF REPEATED SEQUENCES FROM HYBRIDIZATION PROBES
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DOI：
10.1093/nar/13.6.1905
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发表时间：
1985-01-01
影响因子：
14.9
通讯作者：
SOUTHERN, EM
中科院分区：
文献类型：
--
作者：
SEALEY, PG;WHITTAKER, PA;SOUTHERN, EM
含有分散的高度重复序列（例如Alu和Kpn I家族）的人克隆探针与大量过量的超声处理的总人DNA的预再结合允许在转移杂交中检测来自单个和低拷贝数组分的信号。来自非分散重复序列的信号被降低到单拷贝水平。该程序，这是简单和快速的，说明使用模型组合的特点克隆探针，并施加到随机选择的粘粒克隆的样品。理论评估，这可能是有用的那些希望使用这个程序。
Pre-reassociation of human clone probes, containing dispersed highly repeated sequences, (e.g. Alu and KpnI families), with a large excess of sonicated total human DNA allows signal from single and low copy number components to be detected in transfer hybridisations. The signal from non-dispersed repeated sequences is reduced to single copy levels. The procedure, which is simple and quick, is illustrated using model combinations of well characterised cloned probes, and is applied to a sample of randomly chosen cosmid clones. A theoretical assessment is presented which may be useful to those wishing to use this procedure.