Electron flow and heme-heme interaction between cytochromes b-558, b-595 and d in a terminal oxidase of Escherichia coli.
Electron flow and heme-heme interaction between cytochromes b-558, b-595 and d in a terminal oxidase of Escherichia coli.
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大肠杆菌末端氧化酶中细胞色素 b-558、b-595 和 d 之间的电子流和血红素-血红素相互作用。
DOI：
10.1016/0005-2728(87)90050-8
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Y. Anraku
中科院分区：
文献类型：
--
作者：
A. Hata;K. Matsuura;S. Itoh;Y. Anraku
研究了低温下大肠杆菌细胞色素复合体中细胞色素的ESR信号。当细胞色素的电子态由氧化态变为还原态、氧结合态或CO结合态时，菱形高自旋信号的强度和值发生了变化。因此，这些菱形信号被分配给细胞色素B-595，它位于细胞色素附近的氧化酶复合体中。这一分配得到了如下发现的支持，即菱形信号的Em值不同于细胞色素的Em值(Hata，A.等人)。(1985)Biochim。生物群落。学报810，62-72)。在−140℃的氧气存在下，对氧化还原的CO络合物进行了光解和配体交换实验，测定了受控低温捕获的三种中间体的电子自旋共振波谱。这些形式被指定为氧结合中间体I(ESR沉默)、氧结合中间体II(给出ESR信号ATG=6.3、5.5和2.15)和氧结合中间体III(给出信号ATG=6.3、5.5和6.0)。根据这些结果，细胞色素复合体中的电子流被认为是按如下顺序进行的：细胞色素B-558→细胞色素B-595→细胞色素→O2。本文提出了细胞色素络合物氧化泛喹酚-8和生成水的四电子化学机理模型。
The ESR signals of the cytochromes in theEscherichia coliterminal oxidase cytochromedcomplex were studied at cryogenic temperature. The intensities andgvalues of the rhombic high-spin signals changed when the electronic state of cytochromedwas changed from the oxidized state to the reduced or oxygen-binding or CO-binding state. These rhombic signals were therefore assigned to cytochromeb-595, which is located near cytochromedin the oxidase complex. This assignment was supported by the finding that theEmvalue of the rhombic signals differed from that of cytochromed(Hata, A. et al. (1985) Biochim. Biophys. Acta 810, 62–72). Photolysis and ligand-exchange experiments with the reduced CO complex of the oxidase were performed in the presence of oxygen at −140°C. The ESR spectra of three intermediate forms trapped by controlled low temperatures were detected. These forms were designated as the oxygenbinding intermediate I (ESR-silent), oxygen-binding intermediate II (giving ESR signals atg= 6.3, 5.5 and 2.15), and oxygen-binding intermediate III (giving signals atg= 6.3, 5.5 and 6.0). From these results, electron flow in the cytochromedcomplex is proposed to proceed in the order, cytochromeb-558 → cytochromeb-595 → cytochromed→ O2. A model of the mechanism of four-electron chemistry for oxidation of ubiquinol-8 and formation of H2O by the cytochromedcomplex is presented.
鉴定亚基 I 为大肠杆菌细胞色素 d 末端氧化酶复合物的细胞色素 b558 成分。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Green,GN;Kranz,RG;Lorence,RM;Gennis,RB
通讯作者：
Gennis,RB
使用多克隆和单克隆抗体表征大肠杆菌细胞色素 d 末端氧化酶复合物。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kranz,RG;Gennis,RB
通讯作者：
Gennis,RB
对纯化的大肠杆菌细胞色素 d 复合物进行库仑分析和光谱分析：将“细胞色素 a1”鉴定为细胞色素 b595 的证据。
影响因子：
2.9
作者：
Lorence,RM;Koland,JG;Gennis,RB
通讯作者：
Gennis,RB