A comparative account of glucose yields and bioethanol production from separate and simultaneous saccharification and fermentation processes at high solids loading with variable PEG concentration
A comparative account of glucose yields and bioethanol production from separate and simultaneous saccharification and fermentation processes at high solids loading with variable PEG concentration
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DOI：
10.1016/j.biortech.2019.03.060
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发表时间：
2019-07-01
影响因子：
11.4
通讯作者：
Murthy, Ganti S.
中科院分区：
文献类型：
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作者：
Kadhum, Haider Jawad;Mahapatra, Durga Madhab;Murthy, Ganti S.
通过添加聚乙二醇 (PEG6000) 来帮助实现最佳酶水解的工艺策略针对单独水解和发酵 (SHF) 以及同时糖化和发酵 (SSF) 进行了测试。通过 SHF 和 SSF，用 0、0.5、1、1.5、2 和 2.5% 的 PEG6000 对 30% 固体 (w/w) 负载量的预处理麦秆进行酶水解。在 SHF 过程中，生物乙醇浓度达到 107.5 g/L (2.5% PEG6000)。添加 1.5% PEG6000 时，SSF 乙醇浓度约为 113 g/L。技术经济可行性显示，使用 0.5% PEG6000 进行 SHF（96 小时）的投资回报率 (ROI) 为 8.13%，使用 SSF 控制（72 小时）的投资回报率 (ROI) 为 12.25%。对各种情景的生命周期评估表明，SSF 的最佳情况比 SHF 具有更高的环境效益。研究表明，SSF 方法（0% PEG6000；72 小时）有助于提高工艺效率；技术经济收益和高环境可持续性，以实现未来的规模化和商业化。
A process strategy to aid in optimal enzymatic hydrolysis through the addition of polyethylene glycol (PEG6000) was tested for separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). Pretreated wheat straw at 30% solids (w/w) loading was enzymatically hydrolyzed with 0, 0.5, 1, 1.5, 2 and 2.5% of PEG6000 through SHF and SSF. During SHF, bioethanol concentration of 107.5 g/L (2.5% PEG6000) was achieved. SSF ethanol concentration were about 113 g/L at 1.5% PEG6000 addition. A technoeconomic feasibility showed a return on investment (ROI) of 8.13% using 0.5% PEG6000 for SHF (96 h) and 12.25% ROI for SSF control (72 h). Life cycle assessment for the various scenarios indicated higher environmental gains for best cases of SSF over SHF. The study shows the SSF approach (0% PEG6000; 72 h) facilitates higher process efficiencies; technoeconomic gains and high environmental sustainability for future scale-up and commercial realization.