Tissue tropism of a Thailand strain of high-pathogenicity avian influenza virus (H5N1) in tissues of naturally infected native chickens (Gallus gallus), Japanese quail (Coturnix coturnix japonica) and ducks (Anas spp.)
Tissue tropism of a Thailand strain of high-pathogenicity avian influenza virus (H5N1) in tissues of naturally infected native chickens (Gallus gallus), Japanese quail (Coturnix coturnix japonica) and ducks (Anas spp.)
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DOI：
10.1080/03079450600714510
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发表时间：
2006-06-01
期刊：
影响因子：
2.8
通讯作者：
Prommuang, Praison
中科院分区：
文献类型：
--
作者：
Antarasena, Chongmas;Sirimujalin, Rungtiva;Prommuang, Praison
高致病性禽流感H5 N1病毒泰国株的嗜性在来自自然感染的本地鸡（原鸡）、日本鹌鹑（鹌鹑）和鸭（鸭属）的组织（肺、气管、心脏、肝脏、脾脏、胰腺、直肠、肾脏、脑、骨骼肌、十二指肠和输卵管）上得到证实。间接免疫荧光法。在鸡和鹌鹑中，核蛋白病毒抗原的分布和定位相似，在心肌细胞中检测到最高水平，分别为88%（鸡）和89%（鹌鹑），呼吸系统、消化系统和泌尿系统都显示出高水平的抗原。除脑和骨骼肌外，鸭组织中的抗原检测水平显著降低（P < 0.05）。在大多数情况下，鸭组织中的抗原在消化器官中不存在，但在呼吸器官中存在，这支持了气溶胶和口-口传播是高致病性禽流感病毒从该物种传播的主要方式的假设。
The tropism of a Thailand strain of highly pathogenic avian influenza H5N1 virus was demonstrated on tissues ( lung, trachea, heart, liver, spleen, pancreas, rectum, kidney, brain, skeletal muscle, duodenum, and oviduct) from naturally infected native chickens (Gallus gallus), Japanese quail (Coturnix coturnix japonica) and ducks (Anas spp.) by indirect immunofluorescence assay. In chickens and quail, the distribution and localization of nucleoprotein viral antigen was similar and detected at the highest level in cardiac myocytes, at 88% (chickens) and 89% (quail), and respiratory, digestive and urinary systems all showed high levels of antigen. Antigen in duck tissues were detected at significantly lower levels (P < 0.05) with the exception of brain and skeletal muscle samples. In most cases, antigen in duck tissue was absent in the digestive organs but present in respiratory organs, which supports the hypothesis that aerosol and oral-oral transmission are the main method of highly pathogenic avian influenza virus transmission from this species.