Acellular dermal matrix from one-day-old mouse skin on adult scarless cutaneous wound repair by second harmonic generation microscopic imaging
Acellular dermal matrix from one-day-old mouse skin on adult scarless cutaneous wound repair by second harmonic generation microscopic imaging
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DOI：
10.1039/c6ra11179c
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发表时间：
2016-07
期刊：
影响因子：
3.9
通讯作者：
Xue Han;Hanping Liu;Maosheng Chen;Li Gong;Hongwen Pang;Xiaoyuan Deng;Ying Jin
中科院分区：
文献类型：
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作者：
Xue Han;Hanping Liu;Maosheng Chen;Li Gong;Hongwen Pang;Xiaoyuan Deng;Ying Jin
在胎儿皮肤中，有一个无疤痕修复的过程，这在成人中不存在。胎儿皮肤细胞外基质（ECM）的特性被认为在这种无瘢痕伤口愈合中起着关键作用。因此，具有皮肤ECM的某些特定特征的脱细胞真皮基质（ADM）的使用暗示对皮肤修复和再生具有影响，并且ADM性质的改变可以允许开发用于无瘢痕伤口愈合的有效干预。本研究采用两种来源于皮肤细胞外基质的ADM，探讨其在成年小鼠创面无瘢痕愈合中的作用。我们将该基质移植到10周龄的全层皮肤创伤小鼠模型上。选择源自1日龄小鼠皮肤的ECM的ADM（ADM-1D）以接近胎儿皮肤的某些特征，而来自20周龄小鼠皮肤的ECM的ADM（ADM-20 W）提供具有成熟皮肤特征的对照。二次谐波发生（SHG）显微成像动态显示在原发性伤口愈合过程中新生真皮胶原重建过程。评价第21天的愈合结局。与ADM-20 W相比，ADM-1D对表皮重建以及新生真皮中的胶原密度、取向和硬度提供了更有利的影响，达到接近正常未损伤的成人真皮组织的程度。ADM-1D和ADM-20 W之间的生物力学刚度存在显著差异，这可能是潜在的成人无瘢痕伤口愈合的关键决定因素之一。
In fetal skin, there is a process of scarless repair that does not exist in adults. The characteristics of the fetal skin extracellular matrix (ECM) are presumed to play a pivotal role for this scarless wound healing. The use of an acellular dermal matrix (ADM) with certain specific characteristics of the skin ECM thus is implied to have impact on skin repair and regeneration and the alteration of ADM properties may allow for the development of an effective intervention for scarless wound healing. In this study, two types of ADM from skin ECM were adopted to explore the potential to achieve scarless wound healing in adult mice. We transplanted this matrix onto the 10-week-old full-thickness cutaneous wound mice model. The ADM derived from the ECM of 1-day-old mouse skin (ADM-1D) was chosen to approximate certain characteristics of fetal skin while the ADM from the ECM of 20-week-old mouse skin (ADM-20W) provided a control with characteristics of mature skin. Second-harmonic generation (SHG) microscopic imaging was performed to dynamically demonstrate the collagen reconstruction process in the new born dermis during primary wound healing. The outcome of healing on day 21 was evaluated. Compared to ADM-20W, ADM-1D provided a more favorable influence on the re-establishment of the epidermis as well as collagen density, orientation and stiffness in the new born dermis, to a degree approaching normal uninjured adult dermal tissue. A remarkable difference in biomechanical stiffness is present between ADM-1D and ADM-20W, which might be one of the crucial determinants for potential adult scarless wound healing.