Relationship between antimutagenic activity and major components of various teas.
Relationship between antimutagenic activity and major components of various teas.
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DOI：
10.1093/mutage/11.1.37
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发表时间：
1996
期刊：
影响因子：
2.7
通讯作者：
G. Yen;H. Y. Chen
中科院分区：
文献类型：
--
作者：
G. Yen;H. Y. Chen
本研究的目的是确定茶叶和茶叶提取物中的主要成分，并研究各种茶叶提取物的化学含量与抗突变活性之间的关系。儿茶素类物质含量的顺序为：绿色茶（26.7%）>乌龙茶（23.2%）>抱种茶（15.8%）>红茶（4.3%）。乌龙茶提取物中咖啡因和酚类化合物的量分别为8.3%和32.4%，这些量大于其他三种茶提取物。绿色茶萃取物中的抗坏血酸含量略高于乌龙茶和抱种茶。茶叶中儿茶素含量也表现为未发酵茶（绿色茶）>半发酵茶（抱种茶和乌龙茶）>发酵茶（红茶）。乌龙茶中的咖啡因和酚类化合物的含量也高于其他茶叶。除了水溶性成分，茶叶还含有几种脂溶性化学物质，如β-胡萝卜素和生育酚。茶叶提取物，尤其是乌龙茶和普洱茶，对2-氨基-3-甲基咪唑（4，5-f）喹啉（IQ）、3-氨基-1，4-二甲基-5H-吡啶并（4，3-B）吲哚（Trp-P-1）、2-氨基-6-甲基-联吡啶并（1，2-a：3 '，2'-d）咪唑（Glu-P-1）、苯并[a]芘（B[a]P）和黄曲霉毒素B1（AFB 1）的致突变性有明显的抑制作用。茶叶提取物对鼠伤寒沙门氏菌TA 100的IQ和Glu-P-1的诱变作用的抑制作用显示，茶叶提取物对TA 100的B[a]P和AFB 1的诱变作用与茶叶提取物中主要成分的含量呈显著相关（P0.05）。
The objectives of this study were to determine the major components in tea leaves and tea extracts and to study the relationship between chemical content and antimutagenic activity of various tea extracts. The amount of catechins in various tea extracts was in the order: green tea (26.7%) > oolong tea (23.2%) > pouchong tea (15.8%) > black tea (4.3%). The amounts of caffeine and phenolic compounds in oolong tea extracts were 8.3 and 32.4%, respectively; these amounts were greater than those in the other three tea extracts. The ascorbic acid in green tea extracts was a little higher than in oolong and pouchong tea extracts. The amount of catechins in tea leaves also showed the order: nonfermented (green tea) > semifermented (pouchong tea and oolong tea) > fermented tea (black tea). The amounts of caffeine and phenolic compounds in oolong tea leaves are also higher than in other tea leaves. Besides water soluble components, tea leaves also contain several lipid soluble chemicals such as beta-carotene and tocopherols. The tea extracts, especially oolong and pouchong teas, markedly inhibited the mutagenicity of 2-amino-3-methylimidazo (4,5-f)quinoline (IQ), 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indole (Trp-P-1), 2-amino-6-methyl-dipyrido(1,2-a:3',2'-d) imidazole (Glu-P-1), benzo[a]pyrene (B[a]P) and aflatoxin B1 (AFB1). The inhibitory effect of tea extracts against the mutagenicity of IQ and Glu-P-1 in Salmonella typhimurium TA100 showed a significant (P 0.05) correlation was found between the antimutagenicity of tea extracts to B[a]P and AFB1 in TA100 and the content of major components in tea extracts.