In vitro, in vivo studies of a new amphiphilic adsorbent for the removal of low-density lipoprotein.
In vitro, in vivo studies of a new amphiphilic adsorbent for the removal of low-density lipoprotein.
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DOI：
10.1016/s0142-9612(03)00047-4
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发表时间：
2003-06
期刊：
影响因子：
14
通讯作者：
Yan Cheng;Shenqi Wang;Yao-ting Yu;Yi Yuan
中科院分区：
文献类型：
--
作者：
Yan Cheng;Shenqi Wang;Yao-ting Yu;Yi Yuan
根据文献资料（Artif）制备了一种新型两亲性低密度脂蛋白（LDL）吸附剂。细胞，血细胞。, Immob。生物技术，30(4)(2002)285。研究了胆固醇磺化和接枝时间对吸附剂溶胀性能的影响。当胆固醇磺化时间为3 h，接枝时间为5h时，两亲吸附剂对LDL具有较高的吸附能力，对高密度脂蛋白的吸附不明显。该吸附剂对LDL、总胆固醇（TC）和TG的吸附量分别为1.916、2.132、1.349mg/ml。采用高胆固醇或高蛋黄饲料喂养高脂血症兔，然后灌注适量的两亲吸附剂。血液灌流2h后，LDL水平由3.619±0.354降至0.724±0.07mmol/l，说明吸附剂能有效去除LDL，无副作用。
A new amphiphilic adsorbent for the removal of low-density lipoprotein (LDL) was prepared according to the literature (Artif. Cells Blood Subs., Immob. Biotechnol. 30 (4) (2002) 285). The effects of sulfonation and grafting time of cholesterol on the swelling property of adsorbent were studied. When sulfonation and grafting time of cholesterol was 3 and 5h, respectively, the amphiphilic adsorbent had a high adsorption capacity for LDL without significantly adsorbing high-density lipoprotein. The adsorption capacity of the adsorbent for the removal of LDL, total cholesterol (TC) and TG was 1.916, 2.132, 1.349mg/ml, respectively. Hyperlipidemia rabbits were developed by feeding with fodder containing high content of cholesterol or yolk, which was then perfused with an optimal amount of amphiphilic adsorbent. After 2h hemoperfusion, the LDL levels decreased from 3.619±0.354 to 0.724±0.07mmol/l, which showed that the adsorbent could effectively remove LDL without side effect.