Impact of Sampling Type, Frequency, and Scale of the Collection System on SARS-CoV-2 Quantification Fidelity
Impact of Sampling Type, Frequency, and Scale of the Collection System on SARS-CoV-2 Quantification Fidelity
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DOI：
10.1021/acs.estlett.1c00882
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发表时间：
2022-01-18
影响因子：
10.9
通讯作者：
Radniecki, Tyler S.
中科院分区：
文献类型：
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作者：
George, Andrea D.;Kaya, Devrim;Radniecki, Tyler S.
随着新冠肺炎大流行的迅速爆发，基于废水的SARS-CoV2流行病学采样方法往往实施得很快，可能没有考虑到独特的排水集水区特征。本研究在四种不同的集水区尺度上，从高流量(污水处理厂进水)、中流量(社区尺度)、低流量(城市街区尺度)到超低流量(建筑尺度)，评估了Grab和Composal采样对SARS-CoV-2检测和定量的影响。在高流量处，GRAB样品与24小时合成样品具有可比性，所有样品中都检测到SARS-CoV-2，但浓度与合成样品不同
With the rapid onset of the COVID-19 pandemic, wastewater-based epidemiology sampling methodologies for SARS-CoV2 were often implemented quickly and may not have considered the unique drainage catchment characteristics. This study assessed the impact of grab versus composite sampling on the detection and quantification of SARS-CoV-2 in four different catchment scales with flow rates ranging from high flow (wastewater treatment plant influent) to medium flow (neighborhood scale) to low -flow (city block scale) to ultralow flow (building scale). At the high -flow site, grab samples were comparable to 24 h composite samples with SARS-CoV-2 detected in all samples and differed in concentration from the composite by