Changes in protein interactions of cell cycle-related genes during the dormancy-to-growth transition in pea axillary buds.
Changes in protein interactions of cell cycle-related genes during the dormancy-to-growth transition in pea axillary buds.
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豌豆腋芽休眠至生长过渡期间细胞周期相关基因蛋白质相互作用的变化。
DOI：
10.1093/oxfordjournals.pcp.a029304
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发表时间：
1998
影响因子：
4.9
通讯作者：
H. Mori
中科院分区：
文献类型：
--
作者：
Sae Shimizu;H. Mori
顶端优势是顶芽生长占优势而腋芽生长受到抑制的现象。在这里，我们研究了与细胞周期控制相关的分子机制，发生在豌豆腋芽作为一个结果去头。增殖细胞核抗原（PCNA）蛋白在休眠芽和生长芽中均有表达，而PCNA mRNA在休眠芽中无表达。Pissa、CycB 1;2和Cdc 2蛋白在休眠期检测不到。为了分析PCNA和Pissa; CycD 3;1之间的相互作用，我们进行了抗PCNA免疫亲和柱层析。Pissa; CycD 3;1蛋白在休眠芽制备的种子中被检测到，而在生长芽制备的种子中未检测到。此外，我们进行了抗Pissa; CycD 3;1免疫亲和柱层析。PCNA蛋白在休眠芽制备的细胞中被检测到，而在生长芽制备的细胞中未检测到。这些结果表明，PCNA与Pissa; CycD 3;1仅在休眠期相关。此外，PCNA和Pissa; CycD 3;1之间的相互作用被证实的酵母双杂交系统。
Apical dominance is a phenomenon in which a terminal bud grows predominantly and the growth of the axillary buds is suppressed. Here, we investigated the molecular mechanisms associated with cell cycle control that occur in pea axillary buds as a result of decapitation. Proliferating cell nuclear antigen (PCNA) protein was detected in both dormant and growing buds, while PCNA mRNA was absent in dormant buds. Pissa; CycB1;2 and Cdc2 proteins were undetectable during dormancy. To analyze an interaction between PCNA and Pissa;CycD3;1, we performed anti-PCNA immunoaffinity column chromatography. Pissa;CycD3;1 protein was detected in the eluate prepared from the dormant buds, but not in the eluate prepared from the growing buds. Furthermore, we performed anti-Pissa;CycD3;1 immunoaffinity column chromatography. PCNA protein was detected in the eluate prepared from the dormant buds, but not in the eluate prepared from the growing buds. These results indicated that PCNA associated with Pissa;CycD3;1 only during dormancy. In addition, the interaction between PCNA and Pissa;CycD3;1 was confirmed by a yeast two-hybrid system.