Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential
Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential
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DOI：
10.1016/j.biomaterials.2004.10.007
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发表时间：
2005-06-01
期刊：
影响因子：
14
通讯作者：
Matsuda, T
中科院分区：
文献类型：
--
作者：
Kwon, IK;Kidoaki, S;Matsuda, T
采用静电纺丝法制备了具有纳/微结构的可生物降解的聚(L-丙交酯-己内酯)共混物。以二氯甲烷(MC)为溶剂，制备了不同组成(原料摩尔分数为70/30、50150、30/70)、聚L-丙交酯(PLL)、聚c-己内酯(PCL)的超细纤维织物。PLL微纤维具有纳米孔结构，其表面和亚表面区域的孔径约为200-800mN，而在其他含CL的聚合物中很难观察到这种表面结构。PLCL 50/50的超细纤维织物为弹性体。含PlcL 50/50(约0.3或1.2微米)，以1，1，1，3，3，3-六氟-2-丙醇(HFIP)为溶剂。水银测孔仪表明，纤维直径的减小降低了织物的孔隙率，但增加了纤维密度和机械强度。人脐静脉内皮细胞(HUVECs)在小直径纤维织物(直径0.3和1.2微米)上黏附和增殖良好，均为致密纤维织物，而大纤维织物(直径7.0微米)上细胞黏附明显减少，细胞扩散受限，无增殖迹象。(C)2004爱思唯尔有限公司。保留所有权利。
Nano- to micro-structured biodegradable poly(L-lactide-co-epsilon-caprolactone) (PLCL) fabrics were prepared by electrospinning. Electrospun microfiber fabrics with different compositions of PLCL (mol% in feed; 70/30, 50150, and 30/70), poly(L-lactide) (PLL) and poly(c-caprolactone) (PCL) were obtained using methylene chloride (MC) as a solvent. The PLL microfiber exhibited a nanoscale-pore structure with a pore diameter of approximately 200-800 mn at the surface and subsurface regions, whereas such a surface structure was hardly observed in other polymers containing CL. The microfiber fabric made of PLCL 50/50 was elastomeric. Nanoscale-fiber fabrics with PLCL 50/50 (approx. 0.3 or 1.2 mum in diameter) were electrospun using 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) as a solvent. Mercury porosimetry showed that the decrease in the fiber diameter of the fabric decreased porosity, but increased fiber density and mechanical strength. Human umbilical vein endothelial cells (HUVECs) were adhered well and proliferated on the small-diameter-fiber fabrics (0.3 and 1.2 mum in diameter), both of which are dense fabrics, whereas markedly reduced cell adhesion, restricted cell spreading and no signs of proliferation were observed on the large-diameter-fiber fabric (7.0 mum in diameter), The potential biomedical application of electrospun PLCL 50/50 was discussed. (C) 2004 Elsevier Ltd. All rights reserved.