Tracing back EFL gene evolution in the cryptomonads-haptophytes assemblage: Separate origins of EFL genes in haptophytes, photosynthetic cryptomonads, and goniomonads
Tracing back EFL gene evolution in the cryptomonads-haptophytes assemblage: Separate origins of EFL genes in haptophytes, photosynthetic cryptomonads, and goniomonads
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DOI：
10.1016/j.gene.2008.05.010
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发表时间：
2009-07-15
期刊：
影响因子：
3.5
通讯作者：
Inagaki, Yuji
中科院分区：
文献类型：
--
作者：
Sakaguchi, Miako;Takishita, Kiyotaka;Inagaki, Yuji
最近发现的一种GTP酶，延伸因子样蛋白(EFL)，承担翻译延伸因子1α(EF-1α)的主要功能。对EF-1α/EFL进化的开创性研究揭示了EFL在真核生物中的系统发育分散分布，提示了频繁的真核生物到真核生物的EFL基因转移事件以及EFL随后取代了EF-1α的功能。本研究确定了7个新的EFL序列，包括光合作用的隐单胞菌卵形、非光合作用的隐单胞菌、有孔虫PlanogLabratella opecularis、触角类金色藻属、中心鞭毛虫，以及两种红藻Chondrus和Gracilaria changlis。这些EFL序列的分析成功地为真核生物间的EFL基因横向转移提供了新的见解。最令人感兴趣的是由隐单胞体和触须细胞组成的单系组合中复杂的EFL进化。由于我们的分析排除了非单胞菌、光合作用隐单胞菌和单胞菌的EFL序列之间的任何系统发育相似性，这三个谱系的EFL基因很可能来自不同的系统发育来源。(C)2008爱思唯尔B.V.保留所有权利。
A recently identified GTPase, elongation factor-like (EFL) protein is proposed to bear the principal functions of translation elongation factor 1 alpha (EF-1 alpha). Pioneering studies of EF-1 alpha/EFL evolution have revealed the phylogenetically scattered distribution of EFL amongst eukaryotes, suggesting frequent eukaryote-to-eukaryote EFL gene transfer events and subsequent replacements of EF-1 alpha functions by EFL We here determined/identified seven new EFL sequences of the photosynthetic cryptomonad Cryptomonas ovata, the non-photosynthetic cryptomonad (goniomonad) Goniomonas amphinema, the foraminifer Planoglabratella opecularis, the haptophyte Chrysochromulina sp., the centroheliozoan Raphidiophrys contractilis, and two red algae Chondrus crispus and Gracilaria changii. The analyses of these EFL sequences successfully brought new insights into lateral EFL gene transfer amongst eukaryotes. Of most interest is a complex EFL evolution in a monophyletic assemblage comprised of cryptomonads and haptophytes. Since our analyses rejected any phylogenetic affinity amongst the EFL sequences from Goniomonas, photosynthetic cryptomonads, and haptophytes, the EFL genes of the three lineages most likely originated from different phylogenetic sources. (C) 2008 Elsevier B.V. All rights reserved.