Anti-inflammatory and anti-atherogenic effects of cathechin, caffeic acid and trans-resveratrol in apolipoprotein E deficient mice
Anti-inflammatory and anti-atherogenic effects of cathechin, caffeic acid and trans-resveratrol in apolipoprotein E deficient mice
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DOI：
10.1016/j.atherosclerosis.2006.05.047
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发表时间：
2007-04-01
期刊：
影响因子：
5.3
通讯作者：
Catapano, Alberico Luigi
中科院分区：
文献类型：
--
作者：
Norata, Giuseppe Danilo;Marchesi, Patrizia;Catapano, Alberico Luigi
多酚类物质的摄入量与冠心病之间存在很强的负相关性，这一点已被广泛证实。这些结果促进了对多酚类物质在心血管疾病中作用机制的研究。本工作的目的是在apoE基因敲除小鼠中研究P183/1的作用。ApoE基因敲除小鼠喂食补充有P183/1的饮食，40和160 mg/kg体重/天，持续8周。补充高剂量的P183/1显著降低了主动脉窦和升主动脉中动脉粥样硬化斑块的存在，分别为40%和36%。这种减少与巨噬细胞、淋巴细胞（Th 1和Th 2）的标志物以及血管壁中MCP-1、MIP-1 α、MIP-1 β、CCR 1、CCR 2和ET 1的表达减少有关。总之，P183/1补充剂通过影响血管壁中炎性细胞的募集和促炎趋化因子的表达，显著降低ApoE KO小鼠中的动脉粥样硬化。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
A strong negative correlation between polyphenols consumption and coronary heart disease has been extensively documented. These results prompted investigations on the mechanisms responsible for polyphenols effects in cardiovascular disease.The aim of this work was to investigate in apoE KO mice the effect of P183/1 (a mixture of cathechin, caffeic acid and resveratrol) on atherosclerosis and gene expression patterns in the vascular wall.ApoE KO mice were fed a diet supplemented with P183/1, 40 and 160 mg/kg body weight/day for 8 weeks. The supplementation with the high dose of P183/1 significantly reduced the presence of atherosclerotic plaque by 40 and 36% in the aortic sinus and in the ascending aorta, respectively. This reduction was associated with a reduced expression of markers for macrophages, lymphocytes (both Th I and Th2) and of MCP-1, MIP-1 alpha, MIP-1 beta, CCR1, CCR2 and ET1 in the vascular wall. In conclusion, P183/1 supplementation significantly decreases atherosclerosis in ApoE KO mice by affecting inflammatory cells recruitment and expression of pro-inflammatory chemokines in the vascular wall. (c) 2006 Elsevier Ireland Ltd. All rights reserved.