STE20/SPS1-related proline/alanine-rich kinase is involved in plasticity of GABA signaling function in a mouse model of acquired epilepsy.
STE20/SPS1-related proline/alanine-rich kinase is involved in plasticity of GABA signaling function in a mouse model of acquired epilepsy.
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DOI：
10.1371/journal.pone.0074614
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhou L
中科院分区：
文献类型：
--
作者：
Yang L;Cai X;Zhou J;Chen S;Chen Y;Chen Z;Wang Q;Fang Z;Zhou L
The intracellular concentration of chloride ([Cl-]i) determines the strength and polarity of GABA neurotransmission. STE20/SPS1-related proline/alanine-rich kinase (SPAK) is known as an indirect regulator of [Cl-]i for its activation of Na-K-2 Cl-co-transporters (NKCC) and inhibition of K-Cl-co-transporters (KCC) in many organs. NKCC1 or KCC2 expression changes have been demonstrated previously in the hippocampal neurons of mice with pilocarpine-induced status epilepticus (PISE). However, it remains unclear whether SPAK modulates [Cl-]i via NKCC1 or KCC2 in the brain. Also, there are no data clearly characterizing SPAK expression in cortical or hippocampal neurons or confirming an association between SPAK and epilepsy. In the present study, we examined SPAK expression and co-expression with NKCC1 and KCC2 in the hippocampal neurons of mice with PISE, and we investigated alterations in SPAK expression in the hippocampus of such mice. Significant increases in SPAK mRNA and protein levels were detected during various stages of PISE in the PISE mice in comparison to levels in age-matched sham (control) and blank treatment (control) mice. SPAK and NKCC1 expression increased in vitro, while KCC2 was down-regulated in hippocampal neurons following hypoxic conditioning. However, SPAK overexpression did not influence the expression levels of NKCC1 or KCC2. Using co-immunoprecipitation, we determined that the intensity of interaction between SPAK and NKCC1 and between SPAK and KCC2 increased markedly after oxygen-deprivation, whereas SPAK overexpression strengthened the relationships. The [Cl-]i of hippocampal neurons changed in a corresponding manner under the different conditions. Our data suggests that SPAK is involved in the plasticity of GABA signaling function in acquired epilepsy via adjustment of [Cl-]i in hippocampal neurons.
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DOI：
10.1523/jneurosci.1769-10.2010
发表时间：
2010-09-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Dzhala VI;Kuchibhotla KV;Glykys JC;Kahle KT;Swiercz WB;Feng G;Kuner T;Augustine GJ;Bacskai BJ;Staley KJ
通讯作者：
Staley KJ
影响因子：
3.5
作者：
Benini, Ruba;Longo, Daniela;Avoli, Massimo
通讯作者：
Avoli, Massimo
影响因子：
8.3
作者：
Jaenisch, Nadine;Witte, Otto W.;Frahm, Christiane
通讯作者：
Frahm, Christiane
影响因子：
2.9
作者：
Kitamura, Akihiko;Ishibashi, Hitoshi;Nabekura, Junichi
通讯作者：
Nabekura, Junichi
影响因子：
--
作者：
Gagnon, Kenneth B. E.;England, Roger;Delpire, Eric
通讯作者：
Delpire, Eric