Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia
Bone Immune Response to Materials, Part I: Titanium, PEEK and Copper in Comparison to Sham at 10 Days in Rabbit Tibia
复制标题
DOI：
10.3390/jcm7120526
复制
发表时间：
2018-12-01
影响因子：
3.9
通讯作者：
Wennerberg, Ann
中科院分区：
文献类型：
--
作者：
Trindade, Ricardo;Albrektsson, Tomas;Wennerberg, Ann
骨锚定生物材料已成为修复牙体缺失和骨关节置换不可缺少的解决方案。然而，对于导致骨整合的生物学机制以及与之形成对比的种植体周围骨丢失，目前仍缺乏透彻的了解。我们之前曾假设免疫机制参与了这些过程，后来证实钛种植体周围长达4周的骨免疫激活增强。本实验以兔胫骨为模型，比较了钛、聚醚醚酮(PEEK)和铜在愈合10天后的骨炎症/免疫反应，并与假手术对照组进行了比较。在愈合一段时间后，测试和对照部位的样本被处理以进行基因表达分析(聚合酶链式反应，qPCR)和脱钙组织学组织分析。与假手术组相比，所有材料均表现出免疫激活和骨吸收抑制作用。M1(炎症)/M2(修复性)-巨噬细胞表型平衡与种植体周围骨生长的距离和体积相关，钛在10天时表现为M2表型，而铜和PEEK仍处于M1和M2表型的混合环境中。钛是唯一一种显示出足够的骨生长和植入线内接近的材料。10天后，T细胞表面糖蛋白CD4持续上调，T细胞跨膜糖蛋白CD8持续下调，表明所有材料周围均有CD4-淋巴细胞表型驱动反应。
Bone anchored biomaterials have become an indispensable solution for the restoration of lost dental elements and for skeletal joint replacements. However, a thorough understanding is still lacking in terms of the biological mechanisms leading to osseointegration and its contrast, unwanted peri-implant bone loss. We have previously hypothesized on the participation of immune mechanisms in such processes, and later demonstrated enhanced bone immune activation up to 4 weeks around titanium implants. The current experimental study explored and compared in a rabbit tibia model after 10 days of healing time, the bone inflammation/immunological reaction at mRNA level towards titanium, polyether ether ketone (PEEK) and copper compared to a Sham control. Samples from the test and control sites were, after a healing period, processed for gene expression analysis (polymerase chain reaction, (qPCR)) and decalcified histology tissue analysis. All materials displayed immune activation and suppression of bone resorption, when compared to sham. The M1 (inflammatory)/M2 (reparative) -macrophage phenotype balance was correlated to the proximity and volume of bone growth at the implant vicinity, with titanium demonstrating a M2-phenotype at 10 days, whereas copper and PEEK were still dealing with a mixed Ml- and M2-phenotype environment. Titanium was the only material showing adequate bone growth and proximity inside the implant threads. There was a consistent upregulation of (T-cell surface glycoprotein CD4) CD4 and downregulation of (T-cell transmembrane glycoprotein CD8) CD8, indicating a CD4-lymphocyte phenotype driven reaction around all materials at 10 days.