Biogenesis of surface domains during spermiogenesis in the guinea pig.
Biogenesis of surface domains during spermiogenesis in the guinea pig.
复制标题
豚鼠精子发生过程中表面域的生物发生。
DOI：
10.1006/dbio.1993.1012
复制
发表时间：
1993
影响因子：
2.7
通讯作者：
Myles,DG
中科院分区：
文献类型：
--
作者：
Cowan,AE;Myles,DG
在哺乳动物精子发生过程中，球形精子细胞转变为高度不对称的精子细胞。同时，细胞的质膜发育成一个由离散膜区域组成的马赛克，每个区域包含一组独特的蛋白质。通过跟踪精子发生过程中9种不同抗原的表面表达和定位，研究了这些表面结构域的生物发生。每种抗原在睾丸精子上呈现出四种不同定位模式中的一种（全细胞、全头、前尾和后尾），表明睾丸精子上至少有三个不同的表面结构域。我们对抗原定位时间的研究结果表明，哺乳动物精子表面结构域的产生是一个复杂的过程。这一过程涉及抗原表面表达的时间和空间调节，以及抗原到达细胞表面后从不合适的结构域特异性去除。
During mammalian spermiogenesis a spherical spermatid is transformed into a highly asymmetric sperm cell. Concurrently, the plasma membrane of the cell develops into a mosaic of discrete membrane regions, with each region containing a unique set of proteins. Biogenesis of these surface domains was studied by following the surface expression and localization of nine different antigens during spermiogenesis. Each of these antigens exhibits one of four distinct patterns of localization on testicular sperm (whole cell, whole head, anterior tail, and posterior tail), indicating that there are at least three distinct surface domains on testicular sperm. Our results on the timing of antigen localization suggest that the generation of surface domains in mammalian sperm is a complex process. This process involves temporal and spatial regulation of surface expression of the antigens, as well as the specific removal of antigens from inappropriate domains after they have reached the cell surface.