Variation for virulence on Brassica napus L amongst Plasmodiophora brassicae collections from France and derived single-spore isolates
Variation for virulence on Brassica napus L amongst Plasmodiophora brassicae collections from France and derived single-spore isolates
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DOI：
10.1046/j.1365-3059.1996.d01-155.x
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发表时间：
1996-06-01
期刊：
影响因子：
2.7
通讯作者：
Rouxel, F
中科院分区：
文献类型：
--
作者：
Some, A;Manzanares, MJ;Rouxel, F
对法国20个芸苔根肿菌（Plasmodiophora brassicae）的毒力变异进行了研究。我们测试的10个芸苔系中，有7个对接种反应不同;其中。两个油菜品种表现出以前未报道的差异反应。以前用于划分芸苔疫霉致病型的一些差异系对所有收集物都敏感，这表明法国的病原菌群体可能与其他地方报道的不同。利用一组三个甘蓝型油菜品种获得了良好的致病型判别Tt。火灾致病型。P-1、P-2、P-3、P-4和Pg的检出率与寄主类型无关。本文研究了17个单孢菌株的致病性变异，共鉴定出5个致病型。4个菌株被归类为致病型P-1，致病的所有三个差异主机，和8个致病型P-4，致病的三个差异。其他五个菌株被归类为致病型P-3，P-6和P-7，后两个表达模式的反应没有观察到的现场收集。从一个原始孢子悬浮液中分离出不同的致病型个体，证实了芸苔疫霉种群的遗传异质性。
Variation for virulence was examined amongst 20 field collections of Plasmodiophora brassicae from France. Our of the 10 brassica lines tested, seven reacted differentially to inoculation; of these. two oilseed rape cultivars exhibited previously unreported differential responses, Some of the differential lines used previously to classify pathotypes of P. brassicae were susceptible to all collections, suggesting that pathogen populations in France may bs different from those reported elsewhere. Good pathotype discrimination Tt as obtained using a set of three cultivars of Brassica napus. Fire pathotypes. P-1, P-2, P-3, P-4 and Pg, were detected and their occurrence was unrelated to host type. Pathogenic variation amongst 17 single-spore isolates derived from three field collections was studied,:ind five pathotypes were identified. Four isolates were classified as pathotype P-1, pathogenic on all three differential hosts, and eight as pathotype P-4, pathogenic on none of the three differentials. The five other isolates were classified as pathotypes P-3, P-6, and P-7, the latter two expressing patterns of reaction not observed for field collections. Tile fractionation of different individual pathotypes from one original spore suspension confirmed the genetic heterogeneity of held populations of P. brassicae.