Genetic variation and population structure of the Pacific cod Gadus macrocephalus in Korean waters revealed by mtDNA and msDNA markers
Genetic variation and population structure of the Pacific cod Gadus macrocephalus in Korean waters revealed by mtDNA and msDNA markers
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DOI：
10.1007/s12562-011-0403-2
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发表时间：
2011-11-01
影响因子：
1.9
通讯作者：
Nakayama, Kouji
中科院分区：
文献类型：
--
作者：
Gwak, Woo Seok;Nakayama, Kouji
为了研究太平洋大头鳕鱼（Gadus macrocephalus）野生种群之间的遗传分化程度，我们在韩国水域的五个地点检测了遗传多态性[西海保宁（WC-BR）；南海镇海湾（SC-JH）；利用线粒体DNA （mtDNA）控制区和微卫星DNA （msDNA）标记，在韩国东海岸的巨文镇（EC-JM）、竹边镇（EC-JB）和方古镇（EC-BJ）进行了研究。mtDNA控制区5′端可变部分584 bp的核苷酸序列分析显示，184个个体中有27个可变核苷酸位点，分别定义了西部、南部和东部沿海人群的8个、3个和11个单倍型。mtDNA分析显示，韩国水域内这三个地区的种群差异不大，但存在显著的地方差异。msDNA分析也显示了野生群体的中度多态性，5个msDNA标记平均每个位点有13.8-22.6个等位基因，观察到（和预期）杂合度分别为：WC-BR 0.755（0.825）、SC-JH 0.793（0.810）、EC-BJ 0.920（0.905）、EC-JB 0.783（0.865）和EC-JM 0.804（0.812）。结果表明，WC-BR、SC-JH和EC-JB地点的太平洋鳕鱼属于遗传上不同的种群。而SC-JH和EC-BJ的太平洋鳕鱼种群差异不显著。结果表明，通过msDNA分析鉴定出WC-BR、SC-JH、EC-BJ和EC-JB三个遗传差异明显的群体。这些结果表明，遗传上不同的太平洋鳕鱼种群存在于产卵聚集的韩国沿海水域。
To investigate the extent of genetic differentiation among wild populations of the Pacific cod Gadus macrocephalus, we have examined genetic polymorphism at five locations within Korean waters [Boryeong in the West Sea (WC-BR); Jinhae Bay in the South Sea (SC-JH); Jumunjin (EC-JM), Jukbyeon (EC-JB), and Bangeojin (EC-BJ) off the eastern coast of Korea] using mitochondrial DNA (mtDNA) control region and microsatellite DNA (msDNA) markers. Nucleotide sequence analysis of 584 bp in the variable portion of the 5' end of the mtDNA control region revealed 27 variable nucleotide sites among 184 individuals, which defined eight, three, and 11 haplotypes in the western, southern, and eastern coast populations, respectively. The mtDNA analysis revealed a low variability but significant local differentiation among populations from these three areas within Korean waters. msDNA analysis also revealed moderate polymorphism in the wild populations, with a mean of 13.8-22.6 alleles per locus for the five msDNA markers and observed (and expected) heterozygosities of 0.755 (0.825) for the WC-BR, 0.793 (0.810) for the SC-JH, 0.920 (0.905) for the EC-BJ, 0.783 (0.865) for the EC-JB, and 0.804 (0.812) for the EC-JM populations. Analysis of msDNA loci indicated that Pacific cod sampled at the WC-BR, SC-JH, and EC-JB sites belong to genetically distinct populations. However, no significant difference was found between the Pacific cod population from SC-JH and that from EC-BJ. Consequently, three genetically distinct populations, namely, WC-BR, SC-JH and EC-BJ, and EC-JB, were identified using msDNA analysis. These results indicate that genetically distinct populations of Pacific cod are present in Korean coastal waters where spawning aggregations occur.