Synovial fluid mesenchymal stem cells in health and early osteoarthritis - Detection and functional evaluation at the single-cell level
Synovial fluid mesenchymal stem cells in health and early osteoarthritis - Detection and functional evaluation at the single-cell level
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DOI：
10.1002/art.23485
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发表时间：
2008-06-01
影响因子：
--
通讯作者：
McGonagle, Dennis
中科院分区：
文献类型：
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作者：
Jones, Elena A.;Crawford, Aileen;McGonagle, Dennis
客观的。关节炎滑液（SF）含有间充质干细胞（MSC），这可以简单地反映它们从患病关节结构中脱落的情况。本研究使用牛模型探索健康中的 SF MSC，并在放射学正常关节中的人类骨关节炎 (OA) 的早期阶段对它们进行计数。 方法。在单细胞水平上将正常牛 SF MSC 的克隆形成性和多向性与供体匹配的骨髓 (BM) MSC 进行比较。集落形成单位成纤维细胞测定用于 MSC 计数。 XTT法用于评估细胞增殖，流式细胞术用于研究牛和人SF MSC的标记表型。结果。正常牛 SF 中存在单个 MSC，其中 96% 能够扩增至少 100 万倍。这些细胞是 CD271-、多能的、比匹配的 BM MSC 显着更高的克隆形成性和更少的脂肪形成性。在沉淀试验和聚乙醇酸支架上，SF 克隆表现出一致的软骨形成分化，而 BM 克隆则不同。 MSCs 存在于关节镜下正常的人类关节中，并且在早期 OA 中增加了 7 倍（P = 0.034）。它们的数量与游离的微观滑膜组织碎片的数量相关（r = 0.826，P < 0.0001）。 OA SF对滑膜间充质干细胞有促进生长的作用。结论。这项研究证实了正常 SF 中存在 MSC，并显示其在早期人类 OA 中的数量增加。 SF MSC 可能源自滑膜。这些发现为探索 SF MSC 在关节稳态中的潜在作用以及研究其在新型关节再生策略中的效用提供了一个平台。
Objective. Arthritic synovial fluid (SF) contains mesenchymal stem cells (MSCs), which could simply reflect their shedding from diseased joint structures. This study used the bovine model to explore SF MSCs in health and enumerated them at the earliest stages of human osteoarthritis (OA) in radiographically normal joints.Methods. Clonogenicity and multipotentiality of normal bovine SF MSCs were compared with donor-matched bone marrow (BM) MSCs at the single-cell level. The colony-forming unit-fibroblastic assay was used for MSC enumeration. The XTT assay was employed to assess cell proliferation, and flow cytometry was used to investigate the marker phenotype of bovine and human SF MSCs.Results. Single MSCs were present in normal bovine SF, and 96% of them were able to expand at least 1 million-fold. These cells were CD271-, multipotential, considerably more clonogenic, and less adipogenic than matched BM MSCs. In both pellet assays and on polyglycolic acid scaffolds, SF clones displayed consistent chondrogenic differentiation, while BM clones were variable. MSCs were present in arthroscopically normal human joints and were increased 7-fold in early OA (P = 0.034). Their numbers correlated with numbers of free microscopic synovial tissue fragments (r = 0.826, P < 0.0001). OA SF had a growth-promoting effect on synovial MSCs.Conclusion. This study confirms the presence of MSCs in normal SF and shows their numerical increase in early human OA. SF MSCs are likely to originate from synovium. These findings provide a platform for the exploration of the potential role of SF MSCs in joint homeostasis and for investigation of their utility in novel joint regeneration strategies.