Dimethyl Sulfoxide and Ethanol Elicit Increased Amyloid Biogenesis and Amyloid-Integrated Biofilm Formation in Escherichia coli
Dimethyl Sulfoxide and Ethanol Elicit Increased Amyloid Biogenesis and Amyloid-Integrated Biofilm Formation in Escherichia coli
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DOI：
10.1128/aem.07743-11
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发表时间：
2012-05-01
影响因子：
4.4
通讯作者：
Cegelski, Lynette
中科院分区：
文献类型：
--
作者：
Lim, Ji Youn;May, Janine M.;Cegelski, Lynette
大肠杆菌指导功能性淀粉样纤维的组装，这种纤维被称为“卷曲”，介导黏附和生物膜的形成。我们发现，在中等浓度的二甲基亚砜(DMSO)和乙醇的作用下，大肠杆菌在卷曲蛋白表达和纤维组装方面表现出可调节的和选择性的增加。此外，分子变化导致与群落行为相关的戏剧性功能表型，包括(I)肉汤中的细胞凝集，(Ii)菌落形态改变，和(Iii)生物膜形成增加。在[C-13(2)]DMSO存在下形成的完整膜的固体核磁共振谱证实，DMSO没有被转化和直接用于代谢。总的来说，化学诱导的表型强调了大肠杆菌对环境刺激的反应的可塑性，以促进淀粉样蛋白的产生和淀粉样蛋白整合生物膜的形成。这些数据还支持我们将细胞外基质作为包括淀粉样纤维在内的聚合物成分的有组织组装的发展模型，其中的组成与细菌生理和群落功能有关。
Escherichia coli directs the assembly of functional amyloid fibers termed "curli" that mediate adhesion and biofilm formation. We discovered that E. coli exhibits a tunable and selective increase in curli protein expression and fiber assembly in response to moderate concentrations of dimethyl sulfoxide (DMSO) and ethanol. Furthermore, the molecular alterations resulted in dramatic functional phenotypes associated with community behavior, including (i) cellular agglutination in broth, (ii) altered colony morphology, and (iii) increased biofilm formation. Solid-state nuclear magnetic resonance (NMR) spectra of intact pellicles formed in the presence of [C-13(2)] DMSO confirmed that DMSO was not being transformed and utilized directly for metabolism. Collectively, the chemically induced phenotypes emphasize the plasticity of E. coli's response to environmental stimuli to enhance amyloid production and amyloid-integrated biofilm formation. The data also support our developing model of the extracellular matrix as an organized assembly of polymeric components, including amyloid fibers, in which composition relates to bacterial physiology and community function.