Regulation of growth hormone-independent insulin-like growth factor-binding protein (BP-28) in cultured human fetal liver explants.
Regulation of growth hormone-independent insulin-like growth factor-binding protein (BP-28) in cultured human fetal liver explants.
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培养的人胎肝外植体中生长激素独立的胰岛素样生长因子结合蛋白（BP-28）的调节。
DOI：
10.1210/jcem-69-2-246
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
R. Baxter
中科院分区：
文献类型：
--
作者：
M. Lewitt;R. Baxter
使用培养的人胎肝外植体研究GH非依赖性胰岛素样生长因子结合蛋白（BP-28）的调节。BP-28由胎肝外植体在培养的第3天和第6天之间以约200 ng/mg总组织protein.day产生，并且在暴露于放线菌酮（10微克/mL）3天后被抑制60%。20 mmol/L茶碱刺激BP-28产生3倍以上，30 mumol/L毛喉素刺激BP-28产生2倍以上，10 μ g/mL胰高血糖素刺激BP-28产生90%以上。培养液中葡萄糖浓度对BP-28的产生有负调节作用，2 mmol/L葡萄糖组BP-28的产生比30 mmol/L葡萄糖组高68%（P <0.001），而白蛋白的产生比30 mmol/L葡萄糖组低40%（P <0.05）。300 nmol/L胰岛素或3 mmol/L地塞米松可使BP-28水平抑制50%。在培养组织的酸提取物中，BP-28水平低于48小时分泌量的10%。这些结果与细胞内葡萄糖利用率是肝BP-28合成的重要调节剂的结论一致。
Cultured human fetal liver explants were used to study regulation of the GH-independent insulin-like growth factor-binding protein (BP-28). BP-28 was produced by fetal liver explants at approximately 200 ng/mg total tissue protein.day between days 3 and 6 in culture and was inhibited 60% after 3-day exposure to cycloheximide (10 micrograms/mL). BP-28 production was stimulated over 3-fold by 20 mmol/L theophylline, over 2-fold by 30 mumol/L forskolin, and 90% by 10 micrograms/mL glucagon. The glucose concentration in the medium inversely regulated BP-28 production, which was 68% higher in 2 mmol/L glucose than in 30 mmol/L glucose (P less than 0.001); in contrast, albumin production was 40% lower in 2 mmol/L glucose than in 30 mmol/L (P less than 0.05). BP-28 levels were inhibited 50% by 300 nmol/L insulin or 3 mumol/L dexamethasone. In acid extracts of cultured tissue, BP-28 levels were less than 10% of the amount secreted in 48 h. These results are consistent with the conclusion that intracellular glucose availability is an important regulator of hepatic BP-28 synthesis.